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dc.creatorBondžić, Aleksandra M.
dc.creatorLazarević-Pašti, Tamara D.
dc.creatorLeskovac, Andreja R.
dc.creatorPetrović, Sandra
dc.creatorČolović, Mirjana B.
dc.creatorParac-Vogt Tatjana
dc.creatorJanjić, Goran
dc.date.accessioned2020-06-26T10:55:43Z
dc.date.available2020-06-26T10:55:43Z
dc.date.issued2020
dc.identifier.issn0928-0987
dc.identifier.urihttps://cer.ihtm.bg.ac.rs/handle/123456789/3582
dc.description.abstractAcetylcholinesterase (AChE) inhibitors are important in the treatment of neurodegenerative diseases. Two inhibitors, 12-tungstosilicic acid (WSiA) and 12-tungstophosphoric acid (WPA), which have polyoxometalate (POM) type structure, have been shown to inhibit AChE activity in nM concentration. Circular dichroism and tryptophan fluorescence spectroscopy demonstrated that the AChE inhibition was not accompanied by significant changes in the secondary structure of the enzyme. The molecular docking approach has revealed a new allosteric binding site, termed β-allosteric site (β-AS), which is considered responsible for the inhibition of AChE by POMs. To the best of our knowledge, this is the first study reporting a new allosteric site that is considered responsible for AChE inhibition by voluminous and negatively charged molecules such as POMs. The selected POMs were further subjected to genotoxicity testing using human peripheral blood cells as a model system. It was shown that WSiA and WPA induced a mild cytostatic but not genotoxic effects in human lymphocytes, which indicates their potential to be used as medicinal drugs. The identification of non-toxic compounds capable of binding to an allosteric site that so far has not been considered responsible for enzyme inhibition could be fundamental for the development of new drug design strategies and the discovery of more efficient AChE modulators.en
dc.language.isoensr
dc.publisherElseviersr
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200026/RS//sr
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200017/RS//sr
dc.relationCOST action CM1203 Polyoxometalate Chemistry for Molecular Nanoscience (PoCheMoN), ECOST-STSM-CM1203-041015-063147-63147sr
dc.relationCOST action CM1203 Polyoxometalate Chemistry for Molecular Nanoscience (PoCheMoN), COST-STSM-ECOST-STSM-CM1203-030416-072554sr
dc.rightsrestrictedAccesssr
dc.sourceEuropean Journal of Pharmaceutical Sciencessr
dc.subjectAChEsr
dc.subjectNew β-allosteric sitesr
dc.subjectGenotoxicitysr
dc.subjectDocking studysr
dc.subjectPolyoxometalatessr
dc.titleA new acetylcholinesterase allosteric site responsible for binding voluminous negatively charged molecules – the role in the mechanism of AChE inhibitionen
dc.typearticlesr
dc.rights.licenseARRsr
dcterms.abstractБонджић, Aлександра М.; Лазаревић-Паштиа, Тамара Д.; Лесковац, Aндреја Р.; Петровић, Сандра Ж.; Чоловић, Мирјана Б.; Парац-Вогтб, Татјана Н.; Јањић, Горан В.;
dc.rights.holderElseviersr
dc.citation.volume151
dc.citation.spage105376
dc.citation.rankM21~
dc.identifier.pmid32492460
dc.identifier.doi10.1016/j.ejps.2020.105376
dc.identifier.scopus2-s2.0-85085760261
dc.identifier.wos000545890200005
dc.type.versionpublishedVersionsr


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