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dc.creatorGledović, Ana
dc.creatorJanošević Ležaić, Aleksandra
dc.creatorKrstonosic, Veljko
dc.creatorDjokovic, Jelena
dc.creatorNikolić, Ines
dc.creatorBajuk-Bogdanovic, Danica
dc.creatorAntić Stanković, Jelena
dc.creatorRanđelović, Danijela
dc.creatorSavić, Sanela M.
dc.creatorFilipović, Mila
dc.creatorTamburic, Slobodanka
dc.creatorSavić, Snežana D.
dc.date.accessioned2020-05-11T14:11:18Z
dc.date.available2020-05-11T14:11:18Z
dc.date.issued2020
dc.identifier.issn1932-6203
dc.identifier.urihttp://cer.ihtm.bg.ac.rs/handle/123456789/3516
dc.description.abstractConsidering a growing demand for medicinal/cosmetic products with natural actives, this study focuses on the low-energy nanoemulsions (LE-NEs) prepared via the Phase inversion composition (PIC) method at room temperature as potential carriers for natural oil. Four different red raspberry seed oils (ROs) were tested, as follows: cold-pressed vs. CO2-extracted, organic vs. non-organic, refined vs. unrefined. The oil phase was optimized with Tocopheryl acetate and Isostearyl isostearate, while water phase was adjusted with either glycerol or an antioxidant hydro-glycolic extract. This study has used a combined approach to formulation development, employing both conventional methods (pseudo-ternary phase diagram − PTPD, electrical conductivity, particle size measurements, microscopical analysis, and rheological measurements) and the methods novel to this area, such as textural analysis and Raman spectroscopy. Raman spectroscopy has detected fine differences in chemical composition among ROs, and it detected the interactions within nanoemulsions. It was shown that the cold-pressed, unrefined, organic grade oil (RO2) with 6.62% saturated fatty acids and 92.25% unsaturated fatty acids, was optimal for the LE-NEs. Textural analysis confirmed the existence of cubic gel-like phase as a crucial step in the formation of stable RO2-loaded LE-NEs, with droplets in the narrow nano-range (125 to 135 nm; PDI ≤ 0.1). The DPPH test in methanol and ABTS in aqueous medium have revealed a synergistic free radical scavenging effect between lipophilic and hydrophilic antioxidants in LE-NEs. The nanoemulsion carrier has improved the biological effect of raw materials on HeLa cervical adenocarcinoma cells, while exhibiting good safety profile, as confirmed on MRC-5 normal human lung fibroblasts. Overall, this study has shown that low-energy nanoemulsions present very promising carriers for topical delivery of natural bioactives. Raman spectroscopy and textural analysis have proven to be a useful addition to the arsenal of methods used in the formulation and characterization of nanoemulsion systems.
dc.publisherPublic Library of Science (PLoS)en
dc.relationinfo:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/34031/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172043/RS//
dc.relationLondon College of Fashion, University of the Arts London
dc.rightsopenAccess
dc.sourcePLOS ONEen
dc.subjectnanoemulsions
dc.subjectmedicinal/cosmetic products
dc.subjectRaman spectroscopy
dc.subjecttextural analysis
dc.titleLow-energy nanoemulsions as carriers for red raspberry seed oil: Formulation approach based on Raman spectroscopy and textural analysis, physicochemical properties, stability and in vitro antioxidant/ biological activityen
dc.typearticleen
dc.rights.licenseBY
dcterms.abstractЈаносевиц Лезаиц, Aлександра; Крстоносиц, Вељко; Дјоковиц, Јелена; Бајук-Богдановиц, Даница; Aнтиц Станковиц, Јелена; Савиц, Санела М.; Филиповиц, Мила; Николиц, Инес; Савиц, Снезана Д.; Гледовиц, Aна; Тамбуриц, Слободанка; Рандјеловиц, Данијела;
dc.citation.volume15
dc.citation.issue4
dc.citation.spagee0230993
dc.citation.rankM22~
dc.identifier.doi10.1371/journal.pone.0230993
dc.identifier.fulltexthttp://cer.ihtm.bg.ac.rs/bitstream/id/16433/journal.pone.0230993.pdf
dc.identifier.scopus2-s2.0-85083478878
dc.type.versionpublishedVersion


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