Anti-human albumin monoclonal antibody immobilized on EDC-NHS functionalized carboxylic graphene/AuNPs composite as promising electrochemical HSA immunosensor

Abstract

Diagnosis and treatment of some important diseased and metabolic disorders is based on successful detection of albumin. In this work, we aim to develop a simple immunosensor for the detection of human serum albumin in biological fluids. Anti-human albumin antibody was covalently attached to the activated surface of screen-printed carbon electrodes enriched with carboxyl graphene/gold nanoparticles composite. Microstructure (TEM, FE-SEM, XRD) and electrochemical (CV, EIS) characterization methods were used to investigate composite properties and to confirm the successful modification of the electrodes. Under the optimal conditions, linear working range and limit of detection were 2.5–500 μg/mL and 1.55 μg/mL, respectively. Additionally, the effect of some possibly interfering compounds was investigated and the immunosensor was used for real sample analysis. The results showed that the sensor exhibited accurate, precise and sensitive characteristics and can be promising replacement to the convention methods for albumin detection in clinical practice.