Measurements of plasma protein binding – variety of experimental techniques
Konstantinović, Jelena M.
Cvijetić, Ilija N.
Polović, Natalija Đ.
Šolaja, Bogdan A.
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Drug molecules in vivo may be bound to proteins and lipids in plasma and/or in tissues, or free (unbound) in diffusion among the aqueous environment of the blood and tissues. Data from in vitro plasma protein binding experiments that determine the fraction of protein-bound drug are frequently used in drug discovery. Methods used for drug – plasma protein binding (PPB) studies are numerous and can be divided into two main groups: separation methods (enabling the calculation of binding parameters, i.e. the number of binding sites and their respective affinity constants) and non-separation methods (describing predominantly qualitative parameters of the ligand-protein complex).
Keywords:protein binding / in vitro plasma protein binding experiments / drug – plasma protein binding / experimental techniques
Source:6th World Conference on Physico-Chemical Methods in Drug Discovery, Zagreb, Croatia, September 4-7, 2017 - Book of Abstracts, 2017, 30-30
- International Association of Physical Chemists