Measurements of plasma protein binding – variety of experimental techniques
Аутори
Marković, OliveraKonstantinović, Jelena M.
Cvijetić, Ilija
Amézqueta, Susana
Valko, Klara
Ràfols, Clara
Polović, Natalija
Šolaja, Bogdan
Verbić, Tatjana
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Drug molecules in vivo may be bound to proteins and lipids in plasma and/or in tissues, or free (unbound) in diffusion among the aqueous environment of the blood and tissues. Data from in vitro plasma protein binding experiments that determine the fraction of protein-bound drug are frequently used in drug discovery. Methods used for drug – plasma protein binding (PPB) studies are numerous and can be divided into two main groups: separation methods (enabling the calculation of binding parameters, i.e. the number of binding sites and their respective affinity constants) and non-separation methods (describing predominantly qualitative parameters of the ligand-protein complex).
Кључне речи:
protein binding / in vitro plasma protein binding experiments / drug – plasma protein binding / experimental techniquesИзвор:
6th World Conference on Physico-Chemical Methods in Drug Discovery, Zagreb, Croatia, September 4-7, 2017 - Book of Abstracts, 2017, 30-30Издавач:
- International Association of Physical Chemists
Финансирање / пројекти:
- Синтеза аминохинолина и њихових деривата као антималарика и инхибитора ботулинум неуротоксина А (RS-MESTD-Basic Research (BR or ON)-172008)
Институција/група
IHTMTY - CONF AU - Marković, Olivera AU - Konstantinović, Jelena M. AU - Cvijetić, Ilija AU - Amézqueta, Susana AU - Valko, Klara AU - Ràfols, Clara AU - Polović, Natalija AU - Šolaja, Bogdan AU - Verbić, Tatjana PY - 2017 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/3403 AB - Drug molecules in vivo may be bound to proteins and lipids in plasma and/or in tissues, or free (unbound) in diffusion among the aqueous environment of the blood and tissues. Data from in vitro plasma protein binding experiments that determine the fraction of protein-bound drug are frequently used in drug discovery. Methods used for drug – plasma protein binding (PPB) studies are numerous and can be divided into two main groups: separation methods (enabling the calculation of binding parameters, i.e. the number of binding sites and their respective affinity constants) and non-separation methods (describing predominantly qualitative parameters of the ligand-protein complex). PB - International Association of Physical Chemists C3 - 6th World Conference on Physico-Chemical Methods in Drug Discovery, Zagreb, Croatia, September 4-7, 2017 - Book of Abstracts T1 - Measurements of plasma protein binding – variety of experimental techniques SP - 30 EP - 30 UR - https://hdl.handle.net/21.15107/rcub_cer_3403 ER -
@conference{ author = "Marković, Olivera and Konstantinović, Jelena M. and Cvijetić, Ilija and Amézqueta, Susana and Valko, Klara and Ràfols, Clara and Polović, Natalija and Šolaja, Bogdan and Verbić, Tatjana", year = "2017", abstract = "Drug molecules in vivo may be bound to proteins and lipids in plasma and/or in tissues, or free (unbound) in diffusion among the aqueous environment of the blood and tissues. Data from in vitro plasma protein binding experiments that determine the fraction of protein-bound drug are frequently used in drug discovery. Methods used for drug – plasma protein binding (PPB) studies are numerous and can be divided into two main groups: separation methods (enabling the calculation of binding parameters, i.e. the number of binding sites and their respective affinity constants) and non-separation methods (describing predominantly qualitative parameters of the ligand-protein complex).", publisher = "International Association of Physical Chemists", journal = "6th World Conference on Physico-Chemical Methods in Drug Discovery, Zagreb, Croatia, September 4-7, 2017 - Book of Abstracts", title = "Measurements of plasma protein binding – variety of experimental techniques", pages = "30-30", url = "https://hdl.handle.net/21.15107/rcub_cer_3403" }
Marković, O., Konstantinović, J. M., Cvijetić, I., Amézqueta, S., Valko, K., Ràfols, C., Polović, N., Šolaja, B.,& Verbić, T.. (2017). Measurements of plasma protein binding – variety of experimental techniques. in 6th World Conference on Physico-Chemical Methods in Drug Discovery, Zagreb, Croatia, September 4-7, 2017 - Book of Abstracts International Association of Physical Chemists., 30-30. https://hdl.handle.net/21.15107/rcub_cer_3403
Marković O, Konstantinović JM, Cvijetić I, Amézqueta S, Valko K, Ràfols C, Polović N, Šolaja B, Verbić T. Measurements of plasma protein binding – variety of experimental techniques. in 6th World Conference on Physico-Chemical Methods in Drug Discovery, Zagreb, Croatia, September 4-7, 2017 - Book of Abstracts. 2017;:30-30. https://hdl.handle.net/21.15107/rcub_cer_3403 .
Marković, Olivera, Konstantinović, Jelena M., Cvijetić, Ilija, Amézqueta, Susana, Valko, Klara, Ràfols, Clara, Polović, Natalija, Šolaja, Bogdan, Verbić, Tatjana, "Measurements of plasma protein binding – variety of experimental techniques" in 6th World Conference on Physico-Chemical Methods in Drug Discovery, Zagreb, Croatia, September 4-7, 2017 - Book of Abstracts (2017):30-30, https://hdl.handle.net/21.15107/rcub_cer_3403 .