Antitumor effects of a natural anthracycline analog (Aloin) involve altered activity of antioxidant enzymes in HeLaS3 cells
Само за регистроване кориснике
2007
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The anti proliferative and cytotoxic potential of the natural anthracycline aloin from Aloe vera was tested on human uterine carcinoma HeLaS3 cells. Aloin showed a pronounced antiproliferative effect at physiological concentration (IC50 =97 mu M), caused cell cycle arrest in the S phase and markedly increased HeLaS3 cell apoptosis (to 24%). In the concentration range of 20-100 mu M, its action was accompanied by remarkable changes in the activity of almost all antioxidant enzymes: MnSOD activity was increased many fold, while CuZnSOD and iNOS activities were inhibited. Moreover, inhibition of CuZnSOD was shown to occur by direct aloin interaction with the enzyme. As catalase activity was not changed, it is suggested that such conditions were responsible for antiproliferative and cytotoxic effects owing to accumulation of H2O2. Aloin alone was a more potent proapoptotic agent than a 2 Gy fractional dose of ionizing radiation or a combination of the two. Compared to other currently used ...therapeutics, aloin, due to its less undesirable side effects and antimetastatic potential, may prove to be the agent of choice on which clinical protocols for the treatment of human cervical carcinoma should rely in future.
Кључне речи:
aloin / antioxidant enzymes / NF kappa B / HeLaS3 cells / cytotoxicity / cell cycle / proliferation / ionizing radiationИзвор:
Cancer Biology & Therapy, 2007, 6, 8, 1200-1205Издавач:
- Taylor & Francis Inc, Philadelphia
Институција/група
IHTMTY - JOUR AU - Niciforovic, Ana AU - Adžić, Miroslav AU - Spasić, Snežana AU - Radojcic, Marija B. PY - 2007 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/335 AB - The anti proliferative and cytotoxic potential of the natural anthracycline aloin from Aloe vera was tested on human uterine carcinoma HeLaS3 cells. Aloin showed a pronounced antiproliferative effect at physiological concentration (IC50 =97 mu M), caused cell cycle arrest in the S phase and markedly increased HeLaS3 cell apoptosis (to 24%). In the concentration range of 20-100 mu M, its action was accompanied by remarkable changes in the activity of almost all antioxidant enzymes: MnSOD activity was increased many fold, while CuZnSOD and iNOS activities were inhibited. Moreover, inhibition of CuZnSOD was shown to occur by direct aloin interaction with the enzyme. As catalase activity was not changed, it is suggested that such conditions were responsible for antiproliferative and cytotoxic effects owing to accumulation of H2O2. Aloin alone was a more potent proapoptotic agent than a 2 Gy fractional dose of ionizing radiation or a combination of the two. Compared to other currently used therapeutics, aloin, due to its less undesirable side effects and antimetastatic potential, may prove to be the agent of choice on which clinical protocols for the treatment of human cervical carcinoma should rely in future. PB - Taylor & Francis Inc, Philadelphia T2 - Cancer Biology & Therapy T1 - Antitumor effects of a natural anthracycline analog (Aloin) involve altered activity of antioxidant enzymes in HeLaS3 cells VL - 6 IS - 8 SP - 1200 EP - 1205 UR - https://hdl.handle.net/21.15107/rcub_cer_335 ER -
@article{ author = "Niciforovic, Ana and Adžić, Miroslav and Spasić, Snežana and Radojcic, Marija B.", year = "2007", abstract = "The anti proliferative and cytotoxic potential of the natural anthracycline aloin from Aloe vera was tested on human uterine carcinoma HeLaS3 cells. Aloin showed a pronounced antiproliferative effect at physiological concentration (IC50 =97 mu M), caused cell cycle arrest in the S phase and markedly increased HeLaS3 cell apoptosis (to 24%). In the concentration range of 20-100 mu M, its action was accompanied by remarkable changes in the activity of almost all antioxidant enzymes: MnSOD activity was increased many fold, while CuZnSOD and iNOS activities were inhibited. Moreover, inhibition of CuZnSOD was shown to occur by direct aloin interaction with the enzyme. As catalase activity was not changed, it is suggested that such conditions were responsible for antiproliferative and cytotoxic effects owing to accumulation of H2O2. Aloin alone was a more potent proapoptotic agent than a 2 Gy fractional dose of ionizing radiation or a combination of the two. Compared to other currently used therapeutics, aloin, due to its less undesirable side effects and antimetastatic potential, may prove to be the agent of choice on which clinical protocols for the treatment of human cervical carcinoma should rely in future.", publisher = "Taylor & Francis Inc, Philadelphia", journal = "Cancer Biology & Therapy", title = "Antitumor effects of a natural anthracycline analog (Aloin) involve altered activity of antioxidant enzymes in HeLaS3 cells", volume = "6", number = "8", pages = "1200-1205", url = "https://hdl.handle.net/21.15107/rcub_cer_335" }
Niciforovic, A., Adžić, M., Spasić, S.,& Radojcic, M. B.. (2007). Antitumor effects of a natural anthracycline analog (Aloin) involve altered activity of antioxidant enzymes in HeLaS3 cells. in Cancer Biology & Therapy Taylor & Francis Inc, Philadelphia., 6(8), 1200-1205. https://hdl.handle.net/21.15107/rcub_cer_335
Niciforovic A, Adžić M, Spasić S, Radojcic MB. Antitumor effects of a natural anthracycline analog (Aloin) involve altered activity of antioxidant enzymes in HeLaS3 cells. in Cancer Biology & Therapy. 2007;6(8):1200-1205. https://hdl.handle.net/21.15107/rcub_cer_335 .
Niciforovic, Ana, Adžić, Miroslav, Spasić, Snežana, Radojcic, Marija B., "Antitumor effects of a natural anthracycline analog (Aloin) involve altered activity of antioxidant enzymes in HeLaS3 cells" in Cancer Biology & Therapy, 6, no. 8 (2007):1200-1205, https://hdl.handle.net/21.15107/rcub_cer_335 .