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dc.creatorZelenović, Jelena
dc.creatorOstafe, Raluca
dc.creatorFischer, Rainer
dc.creatorProdanović, Radivoje
dc.date.accessioned2019-12-19T14:51:37Z
dc.date.available2019-12-19T14:51:37Z
dc.date.issued2017
dc.identifier.urihttp://www.bds.org.rs/en/conferences.php
dc.identifier.urihttp://cer.ihtm.bg.ac.rs/handle/123456789/3282
dc.description.abstractCellulase (EC 3.2.1.4) are important enzymes in food, paper, textile, detergent and biofuel industries. Most cellulases have low activity and stability. Improving these properties would have substantial impact on numerous industrial processes. Enzymatic properties can be improved by directed evolution, but the screening process is the limiting step. Coupled cellulase assay has been developed in order to improve the screening process. This method does not require boiling samples and allows rapid screening of mutants in a microtiter plate. The aim of this study was to establish enzyme coupled assay where cellulase first hydrolyzes carboxymethylcellulose (CMC), and cellobioses dehydrogenase (CBDH) and dichlorophenolindophenol (DCPIP) is used subsequently for detection of reducing ends.sr
dc.language.isoensr
dc.publisherFaculty of Chemistry, Serbian Biochemical Societysr
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46010/RS//sr
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/43009/RS//
dc.rightsopenAccesssr
dc.sourceSerbian Biochemical Society Seventh Conference "Biochemistry of Control in Life and Technology" - Proceedingssr
dc.subjectCellulasesr
dc.subjectsemi-rational designsr
dc.subjectmutationssr
dc.titleDirected evolution of cellulase from Trichoderma reesei for higher activity and development of microtiter plate assay based on cellobiose dehydrogenasesr
dc.typeconferenceObjectsr
dc.rights.licenseBY-NC-NDsr
dcterms.abstractФисцхер, Раинер; Зеленовић, Јелена; Остафе, Ралуца; Продановић, Радивоје;
dc.identifier.fulltexthttp://cer.ihtm.bg.ac.rs/bitstream/id/15654/04_biochem2017.pdf
dc.type.versionpublishedVersionsr


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