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dc.creatorMilojkov, Dušan V.
dc.creatorSilvestre, Oscar F.
dc.creatorStanić, Vojislav Dj.
dc.creatorJanjić, Goran
dc.creatorMutavdžić, Dragosav
dc.creatorMilanović, Marija
dc.creatorNieder, Jana B.
dc.date.accessioned2019-12-19T13:54:38Z
dc.date.available2021-09-19
dc.date.issued2020
dc.identifier.issn0022-2313
dc.identifier.urihttps://cer.ihtm.bg.ac.rs/handle/123456789/3276
dc.description.abstractFluorapatite doped with rare-earth elements has a wide-range of biomedical applications. Here, a new type of fluorapatite nanocrystals doped with praseodymium (FAP-Pr) with excitation-emission profiles in visible part of the spectrum is fabricated. Energy levels of Pr3+ activator ion contain metastable multiplet states that offer the possibility of efficient multicolor emission lines in FAP nanocrystals. Three types of FAP-Pr nanocrystals with 0.1%, 0.5% and 1% atomic percent of Pr3+ (along with the undoped FAP control sample) are studied. Their novel chemical production method is described, the FAP-Pr nanocrystals structure, biocompatibility and the suitability for cell imaging are analyzed. Physicochemical characterization confirms crystals down to nanometer size. In addition, quantum-chemical calculation predicts that Pr3+ ions are incorporated into the FAP crystal lattice at Ca2 (6 h) sites. In vitro viability results shows that FAP-Pr nanocrystals are nontoxic to live cells. Additionally, the cell uptake of the FAP-Pr nanocrystals is studied using fluorescence-based widefield and confocal microscopy. The nanocrystals show characteristic green emission at 545 nm (3P0→3H5 transition of Pr3+ ion) and orange emission at 600 nm (1D2→3H4), which we use to discriminate from cell autofluorescence background. Orthogonal projections across 3D confocal stacks show that the nanocrystals are able to enter the cells positioning themselves within the cytoplasm. Overall, the new FAP-Pr nanocrystals are biocompatible and of the tested types, the 0.5% Pr3+ doped nanocrystals show the highest promise as a tracking nanoparticle probe for bioimaging applications.en
dc.publisherElsevieren
dc.relationCCDR-N: NORTE-01-0145-FEDER-000019en
dc.relationMarie Curie fellowship, EU-EC, COFUND programme: NanoTRAINforGrowth (600375)en
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/43009/RS//en
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/45021/RS//en
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172023/RS//en
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173017/RS//en
dc.rightsembargoedAccess
dc.sourceJournal of Luminescenceen
dc.subjectFluorapatite nanocrystals
dc.subjectPraseodymium
dc.subjectBioimaging
dc.subjectCancer cells
dc.titleFabrication and characterization of luminescent Pr3+ doped fluorapatite nanocrystals as bioimaging contrast agentsen
dc.typearticleen
dc.rights.licenseARR
dcterms.abstractСтанић, Војислав Дј.; Милојков, Душан В.; Силвестре, Осцар Ф.; Јањић, Горан; Мутавджић, Драгосав Р.; Милановић, Марија; Ниедер, Јана Б.;
dc.rights.holderElsevier
dc.citation.volume217
dc.citation.spage116757
dc.citation.rankM21~
dc.description.otherThis is the peer-reviewed version of the manuscript: Journal of Luminescence, 2020, 217, 116757 [https://doi.org/10.1016/j.jlumin.2019.116757]
dc.description.otherThe published version: [http://cer.ihtm.bg.ac.rs/handle/123456789/3275]
dc.identifier.doi10.1016/j.jlumin.2019.116757
dc.identifier.fulltexthttps://cer.ihtm.bg.ac.rs/bitstream/id/15648/1-s2.0-S0022231319311238-ACC.pdf
dc.identifier.scopus2-s2.0-85073162138
dc.identifier.wos000501362400035
dc.type.versionacceptedVersion


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