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dc.creatorStojićević, Aleksandra S.
dc.creatorPastor, Ferenc T.
dc.creatorGorjanović, Stanislava Ž.
dc.creatorŠolević Knudsen, Tatjana
dc.creatorAntić, Mališa
dc.date.accessioned2019-12-15T11:52:43Z
dc.date.available2019-12-15T11:52:43Z
dc.date.issued2020
dc.identifier.issn0882-5734
dc.identifier.issn1099-1026
dc.identifier.urihttps://cer.ihtm.bg.ac.rs/handle/123456789/3238
dc.description.abstractA direct current (DC) polarographic assay based on the decrease of anodic current of HydroxoPerhydroxoMercury (II) Complex (HPMC) formation in H2O2 solution in Clark Lubs (CL) buffer (pH 9.8) as working solution was previously applied to determine antioxidant (AO) capacity of water‐soluble compounds. Here, the applicability of HPMC assay was extended into samples poorly soluble in water such as the essential oils and extracts of Lamiaceae and Apiaceae species obtained by ultrasound‐assisted maceration (UAM) and Soxhlet extraction (SE) with 70 and 96% ethanol as well as individual compounds present. The influence of solvents miscible with water on HPMC anodic current was studied, and working solution was optimized. A modified HPMC method was applied to measure AO capacity of essential oils and extracts as well as compounds identified using gas chromatography‐mass spectrometer (GC‐MS) and gas chromatography with flame‐ionization detection (GC‐FID) (terpenoids and catechols) or known to be present (phenolic acids and flavonoids). A 1:1 mixture of CL buffer and ethanol was chosen as working solution. AO capacity of essential oils and extracts, established by modified HPMC assay, was in range of 1.2‐2.0 and 9.2‐38.4 (%/mL) while terpenoids and phenolics in range of 0.1‐0.5 and 13.5‐38.5 (%/mol), respectively. The superior AO capacity was ascribed to essential oils and extracts of Lamiaceae species and to extracts obtained with 70% ethanol. A higher AO capacity of extracts in comparison to essential oils was corroborated with a difference in phenolics and terpenoids capacity.en
dc.publisherWileyen
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/43010/RS//
dc.rightsrestrictedAccess
dc.sourceFlavour and Fragrance Journalen
dc.subjectPolarography
dc.subjectAntioxidant capacity
dc.subjectdirect current polarography
dc.subjectGC-FID
dc.subjectGC-MS
dc.subjectHydroxoPerhydroxoMercury(II) complex
dc.titleModification of DC polarographic antioxidant assay—Application to aromatic plants and their active principlesen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractГорјановић, Станислава Ж.; Пастор, Ференц Т.; Шолевић Кнудсен, Татјана; Aнтић, Малиша П.; Стојићевић, Aлександра С.;
dc.citation.volume35
dc.citation.spage219
dc.citation.epage226
dc.citation.rankM22~
dc.identifier.doi10.1002/ffj.3555
dc.identifier.scopus2-s2.0-85075458236
dc.identifier.wos000497127900001
dc.type.versionpublishedVersion


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Приказ основних података о документу