Show simple item record

dc.creatorCvetković, Mirjana
dc.creatorDamjanović, Ana
dc.creatorStanojković, Tatjana
dc.creatorĐorđević, Iris
dc.creatorTešević, Vele
dc.creatorMilosavljević, Slobodan
dc.creatorGođevac, Dejan
dc.date.accessioned2019-09-04T10:00:09Z
dc.date.available2019-09-04T10:00:09Z
dc.date.issued2020
dc.identifier.issn0039-9140
dc.identifier.urihttp://cer.ihtm.bg.ac.rs/handle/123456789/3054
dc.description.abstractMetabolomics generate a profile of small molecules from plant extracts, which could be directly responsible for bioactivity effects. Using dry-column flash chromatography enabled a rapid and inexpensive method for the very efficient separation of plant extract with a high resolution. This separation method coupled to NMR and FTIR based metabolomics is applied to identify bioactive natural products. OPLS multivariate analysis method, was used for correlation the chemical composition of the plant extracts, Amphoricarpos autariatus, with the results of cytotoxic activity against Human cervical adenocarcinoma cell line (HeLa) and epithelial lung cancer cell line (A549). In this way, the highest contribution to the cytotoxic activity was recorded for the guaianolide sesquiterpene lactones named amphoricarpolides. The compounds indicated as bioactive after metabolomics analysis were tested, and their cytotoxic activity were confirmed.sr
dc.language.isoensr
dc.publisherElseviersr
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172053/RS//sr
dc.rightsrestrictedAccesssr
dc.sourceTalantasr
dc.subjectAmphoricarpos autariatussr
dc.subjectNMRsr
dc.subjectFTIRsr
dc.subjectMetabolomicssr
dc.subjectCytotoxic activitysr
dc.subjectDry-column flash chromatographysr
dc.titleIntegration of dry-column flash chromatography with NMR and FTIR metabolomics to reveal cytotoxic metabolites from Amphoricarpos autariatussr
dc.typearticlesr
dc.rights.licenseARRsr
dcterms.abstractТешевић, Веле; Ђорђевић, Ирис; Станојковић, Татјана П.; Дамјановић, Aна; Цветковић, Мирјана; Милосављевић, Слободан; Гођевац, Дејан;
dc.rights.holderElseviersr
dc.citation.volume206
dc.citation.spage120248
dc.citation.rankM21~
dc.identifier.doi10.1016/j.talanta.2019.120248
dc.identifier.scopus2-s2.0-85070705746
dc.identifier.wos000488416200070
dc.type.versionpublishedVersionsr


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record