Acidic horseradish peroxidase activity abolishes genotoxicity of common dyes
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The aim of this study was to investigate the impact of dyes on DNA before and after enzymatic decolorization by acidic horseradish peroxidase (HRP-A). The comet assay is easy and feasible method widely used to measure DNA damage and repair. The medium-throughput comet assay was employed for assessment of genotoxic effects of 8 dyes in BEAS-2B cells. We have incorporated a digestion with bacterial endonuclease (formamidopyrimidine DNA glycosylase, FPG) to detect oxidized bases in the case of single and double azo dyes, Orange II (OR2) and Amido Black 10B (AB), respectively. This allowed detection 8-oxo7,8-dihydroguanine, one of most abundant oxidized bases in nuclear DNA. In the case of AB there was no indication of DNA damage, either strand brakes or FPG-sensitive sites before and after decolorization. The OR2 induced DNA damage (in terms of percentage of DNA in comet tails). Also, the frequency of FPG-sensitive sites increased with OR2 concentration. After decolorization no DNA damagi...ng effects was seen at all. The interaction studies of OR2 and AB, before and after decolorization, with calf thymus DNA has been investigated by absorption and fluorescence spectroscopy. The results provide support for the idea that in some cases enzymatic decolorization contributes to lower genotoxicity potential.
Keywords:
DNA damage / Comet assay / Horseradish peroxidase a / Decolorization / Dye degradation / DNA interactionsSource:
Journal of Hazardous Materials, 2017, 321, 576-585Publisher:
- Elsevier
Funding / projects:
- Production, purification and characterization of enzymes and small molecules and their application as soluble or immobilized in food biotechnology, biofuels production and environmental protection (RS-172048)
- FEBS Collaborative Experimental Scholarship, Oslo, Norway
Note:
- This is peer-reviewed version of the artcle: B.S. Janović, A.R. Collins, Z.M. Vujčić, M.T. Vujčić, Acidic horseradish peroxidase activity abolishes genotoxicity of common dyes, J. Hazard. Mater. 321 (2017) 576–585. https://doi.org/10.1016/j.jhazmat.2016.09.037
- The published version: http://cer.ihtm.bg.ac.rs/handle/123456789/2070
DOI: 10.1016/j.jhazmat.2016.09.037
ISSN: 0304-3894
PubMed: 27694021
WoS: 000388777300061
Scopus: 2-s2.0-84988963283
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IHTMTY - JOUR AU - Janović, Barbara AU - Collins, Andrew R. AU - Vujčić, Zoran AU - Vujčić, Miroslava PY - 2017 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/3045 AB - The aim of this study was to investigate the impact of dyes on DNA before and after enzymatic decolorization by acidic horseradish peroxidase (HRP-A). The comet assay is easy and feasible method widely used to measure DNA damage and repair. The medium-throughput comet assay was employed for assessment of genotoxic effects of 8 dyes in BEAS-2B cells. We have incorporated a digestion with bacterial endonuclease (formamidopyrimidine DNA glycosylase, FPG) to detect oxidized bases in the case of single and double azo dyes, Orange II (OR2) and Amido Black 10B (AB), respectively. This allowed detection 8-oxo7,8-dihydroguanine, one of most abundant oxidized bases in nuclear DNA. In the case of AB there was no indication of DNA damage, either strand brakes or FPG-sensitive sites before and after decolorization. The OR2 induced DNA damage (in terms of percentage of DNA in comet tails). Also, the frequency of FPG-sensitive sites increased with OR2 concentration. After decolorization no DNA damaging effects was seen at all. The interaction studies of OR2 and AB, before and after decolorization, with calf thymus DNA has been investigated by absorption and fluorescence spectroscopy. The results provide support for the idea that in some cases enzymatic decolorization contributes to lower genotoxicity potential. PB - Elsevier T2 - Journal of Hazardous Materials T1 - Acidic horseradish peroxidase activity abolishes genotoxicity of common dyes VL - 321 SP - 576 EP - 585 DO - 10.1016/j.jhazmat.2016.09.037 ER -
@article{ author = "Janović, Barbara and Collins, Andrew R. and Vujčić, Zoran and Vujčić, Miroslava", year = "2017", abstract = "The aim of this study was to investigate the impact of dyes on DNA before and after enzymatic decolorization by acidic horseradish peroxidase (HRP-A). The comet assay is easy and feasible method widely used to measure DNA damage and repair. The medium-throughput comet assay was employed for assessment of genotoxic effects of 8 dyes in BEAS-2B cells. We have incorporated a digestion with bacterial endonuclease (formamidopyrimidine DNA glycosylase, FPG) to detect oxidized bases in the case of single and double azo dyes, Orange II (OR2) and Amido Black 10B (AB), respectively. This allowed detection 8-oxo7,8-dihydroguanine, one of most abundant oxidized bases in nuclear DNA. In the case of AB there was no indication of DNA damage, either strand brakes or FPG-sensitive sites before and after decolorization. The OR2 induced DNA damage (in terms of percentage of DNA in comet tails). Also, the frequency of FPG-sensitive sites increased with OR2 concentration. After decolorization no DNA damaging effects was seen at all. The interaction studies of OR2 and AB, before and after decolorization, with calf thymus DNA has been investigated by absorption and fluorescence spectroscopy. The results provide support for the idea that in some cases enzymatic decolorization contributes to lower genotoxicity potential.", publisher = "Elsevier", journal = "Journal of Hazardous Materials", title = "Acidic horseradish peroxidase activity abolishes genotoxicity of common dyes", volume = "321", pages = "576-585", doi = "10.1016/j.jhazmat.2016.09.037" }
Janović, B., Collins, A. R., Vujčić, Z.,& Vujčić, M.. (2017). Acidic horseradish peroxidase activity abolishes genotoxicity of common dyes. in Journal of Hazardous Materials Elsevier., 321, 576-585. https://doi.org/10.1016/j.jhazmat.2016.09.037
Janović B, Collins AR, Vujčić Z, Vujčić M. Acidic horseradish peroxidase activity abolishes genotoxicity of common dyes. in Journal of Hazardous Materials. 2017;321:576-585. doi:10.1016/j.jhazmat.2016.09.037 .
Janović, Barbara, Collins, Andrew R., Vujčić, Zoran, Vujčić, Miroslava, "Acidic horseradish peroxidase activity abolishes genotoxicity of common dyes" in Journal of Hazardous Materials, 321 (2017):576-585, https://doi.org/10.1016/j.jhazmat.2016.09.037 . .