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dc.creatorLončarević, Branka D.
dc.creatorLješević, Marija
dc.creatorMarković, Marijana
dc.creatorAnđelković, Ivan
dc.creatorGojgić-Cvijović, Gordana
dc.creatorJakovljević, Dragica
dc.creatorBeškoski, Vladimir
dc.date.accessioned2019-06-28T14:08:53Z
dc.date.available2019-06-28T14:08:53Z
dc.date.issued2019
dc.identifier.issn0147-6513
dc.identifier.urihttp://cer.ihtm.bg.ac.rs/handle/123456789/2959
dc.description.abstractMicrobial polysaccharides, due to their unique physiochemical properties, have found application in the food industry, cosmetics, pharmacy and medicine. In the environment, microbes can use polysaccharides to alleviate the adverse effects of heavy metals in their close proximity. This adaptive property shows interesting potential for bioremediation. Herein, the effects of the exopolysaccharides (EPS) levan, produced by the bacterium Bacillus licheniformis NS032 and pullulan, produced by the fungus Aureobasidium pullulans CH-1 in the presence of copper (Cu2+) have been investigated for the first time on antioxidant enzyme activity, respiration and Cu2+ bioaccumulation of Daphnia magna as well as the bioluminescence of Vibrio fischeri. Both EPS decreased toxicity of Cu2+ in the acute test with D. magna. The activity of catalase (CAT) was significantly diminished after acute exposure to Cu2+ in comparison to treatments with Cu2+ and EPS, while in the prolonged acute exposure the CAT activity did not show statistically significant (P ≤ 0.05) differences between treatments with and without the EPS. According to ICP-MS results, during prolonged acute exposure of neonates, the bioaccumulation of Cu2+ in treatments without the EPS was 52.03 μg/g of biomass (wet), while in treatments with EPS, the bioaccumulation was lower by one order of magnitude. The respiration of neonates during acute exposure to Cu2+ with or without the EPS was monitored using the MicroOxymax respirometer, and the results show the EPS can positively effect the respiration. In the case of bacterial bioluminescence, the toxicity of Cu2+ decreased in treatments with EPS (30 min EC10) from 3.54 mg/L to 140.61 mg/L (levan) and 45.00 mg/L (pullulan). This study demonstrates protective effect of EPS against Cu2+ toxicity on D. magna and V. fischeri, and opens the door for further investigation of potential application of levan and pullulan in bioremediation of heavy metals and mitigation of their adverse effects in the aquatic environment.
dc.publisherElsevier
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/43004/RS//
dc.rightsrestrictedAccess
dc.sourceEcotoxicology and Environmental Safety
dc.subjectCopper
dc.subjectD. magna
dc.subjectLevan
dc.subjectPullulan
dc.subjectRespirometer
dc.subjectV. fischeri
dc.titleMicrobial levan and pullulan as potential protective agents for reducing adverse effects of copper on Daphnia magna and Vibrio fischeri
dc.typearticle
dc.rights.licenseARR
dcterms.abstractБешкоски, Владимир; Лончаревић, Бранка Д.; Љешевић, Марија; Aнђелковић, Иван Б.; Гојгић-Цвијовић, Гордана Д.; Јаковљевић, Драгица М.; Марковић, Маријана; Мицробиал леван анд пуллулан ас потентиал протецтиве агентс фор редуцинг адверсе еффецтс оф цоппер он Дапхниа магна анд Вибрио фисцхери;
dc.citation.volume181
dc.citation.spage187
dc.citation.epage193
dc.citation.rankM21~
dc.identifier.doi10.1016/j.ecoenv.2019.06.002
dc.identifier.scopus2-s2.0-85066956975
dc.identifier.wos000475411500022
dc.type.versionpublishedVersion


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