Biodegradation of Isoprenoids, Steranes, Terpanes, and Phenanthrenes During In Situ Bioremediation of Petroleum-Contaminated Groundwater
Autori
Beškoski, VladimirMiletić, Srđan
Ilić, Mila
Gojgić-Cvijović, Gordana
Papić, Petar
Marić, Nenad
Šolević Knudsen, Tatjana
Jovančićević, Branimir
Nakano, Takeshi
Vrvić, Miroslav
Članak u časopisu (Recenzirana verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The objective of this study was to stimulate microbial biodegradation of petroleum pollution in groundwater and to analyze changes in the abundance and distribution of organic compounds detectable in petroleum. Bioremediation was conducted in a closed bipolar system, by bioaugmentation with consortia of hydrocarbon degrading microorganisms (HD) and biostimulation with nutrients. Comprehensive twodimensional gas chromatography-mass spectrometry (GC X GC-MS) was used to visualize all fractions simultaneously. During the study, the content of total petroleum hydrocarbon (TPH) in groundwater decreased by 92.7% of the initial level, and the average rate of biodegradation was 0.1 mg/L per day. Increased numbers of HD were observed and the dominant genera were Pseudomonas, Rhodococcus, Achromobacter, Bacillus, and Micromonospora. In the first 30 days of bioremediation, there was no significant biodegradation of n-alkanes and petroleum biomarkers - isoprenoids such as pristane and phytane, and... polycyclic-saturated hydrocarbons such as terpanes and steranes. However, after 60 days of bioremediation, more than 95% of n-alkanes, terpanes and steranes were biodegraded. Phenanthrene and its methyl-,dimethyl-, and trimethyl-isomers were biodegraded and reduced by more than 99% of their initial levels. However, their decomposition had clearly commenced after just 30 days. This is a somewhat surprising result since it follows that the phenanthrenes were more susceptible to biodegradation than the n-alkanes and isoprenoids. Depending on the microbial community used for bioaugmentation, biodegradation of phenanthrene can precede biodegradation of saturated hydrocarbons.
Ključne reči:
Closed bipolar systems / Petroleum biomarkers / Polycyclic aromatic hydrocarbons / Two-dimensional gas chromatography–mass spectrometry / Zymogenous microorganismsIzvor:
Clean - Soil, Air, Water, 2017, 45, 2Izdavač:
- Wiley, Hoboken
Finansiranje / projekti:
- Simultana bioremedijacija i soilifikacija degradiranih prostora, za očuvanje prirodnih resursa biološki aktivnih supstanci i razvoj i proizvodnju biomaterijala i dijetetskih proizvoda (RS-43004)
- Geohemijska ispitivanja sedimentnih stena - fosilna goriva i zagađivači životne sredine (RS-176006)
- Japan International Cooperation Agency (JICA)
Napomena:
- This is the peer-reviewed version of the following article: Beškoski, V. P.; Miletić, S.; Ilić, M.; Gojgić-Cvijović, G.; Papić, P.; Marić, N.; Šolević-Knudsen, T.; Jovančićević, B. S.; Nakano, T.; Vrvić, M. M. Biodegradation of Isoprenoids, Steranes, Terpanes, and Phenanthrenes During In Situ Bioremediation of Petroleum-Contaminated Groundwater. Clean - Soil, Air, Water 2017, 45 (2). https://doi.org/10.1002/clen.201600023
- http://cer.ihtm.bg.ac.rs/handle/123456789/2183
DOI: 10.1002/clen.201600023
ISSN: 1863-0650
WoS: 000397036000010
Scopus: 2-s2.0-85010284829
Institucija/grupa
IHTMTY - JOUR AU - Beškoski, Vladimir AU - Miletić, Srđan AU - Ilić, Mila AU - Gojgić-Cvijović, Gordana AU - Papić, Petar AU - Marić, Nenad AU - Šolević Knudsen, Tatjana AU - Jovančićević, Branimir AU - Nakano, Takeshi AU - Vrvić, Miroslav PY - 2017 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/2925 AB - The objective of this study was to stimulate microbial biodegradation of petroleum pollution in groundwater and to analyze changes in the abundance and distribution of organic compounds detectable in petroleum. Bioremediation was conducted in a closed bipolar system, by bioaugmentation with consortia of hydrocarbon degrading microorganisms (HD) and biostimulation with nutrients. Comprehensive twodimensional gas chromatography-mass spectrometry (GC X GC-MS) was used to visualize all fractions simultaneously. During the study, the content of total petroleum hydrocarbon (TPH) in groundwater decreased by 92.7% of the initial level, and the average rate of biodegradation was 0.1 mg/L per day. Increased numbers of HD were observed and the dominant genera were Pseudomonas, Rhodococcus, Achromobacter, Bacillus, and Micromonospora. In the first 30 days of bioremediation, there was no significant biodegradation of n-alkanes and petroleum biomarkers - isoprenoids such as pristane and phytane, and polycyclic-saturated hydrocarbons such as terpanes and steranes. However, after 60 days of bioremediation, more than 95% of n-alkanes, terpanes and steranes were biodegraded. Phenanthrene and its methyl-,dimethyl-, and trimethyl-isomers were biodegraded and reduced by more than 99% of their initial levels. However, their decomposition had clearly commenced after just 30 days. This is a somewhat surprising result since it follows that the phenanthrenes were more susceptible to biodegradation than the n-alkanes and isoprenoids. Depending on the microbial community used for bioaugmentation, biodegradation of phenanthrene can precede biodegradation of saturated hydrocarbons. PB - Wiley, Hoboken T2 - Clean - Soil, Air, Water T1 - Biodegradation of Isoprenoids, Steranes, Terpanes, and Phenanthrenes During In Situ Bioremediation of Petroleum-Contaminated Groundwater VL - 45 IS - 2 DO - 10.1002/clen.201600023 ER -
@article{ author = "Beškoski, Vladimir and Miletić, Srđan and Ilić, Mila and Gojgić-Cvijović, Gordana and Papić, Petar and Marić, Nenad and Šolević Knudsen, Tatjana and Jovančićević, Branimir and Nakano, Takeshi and Vrvić, Miroslav", year = "2017", abstract = "The objective of this study was to stimulate microbial biodegradation of petroleum pollution in groundwater and to analyze changes in the abundance and distribution of organic compounds detectable in petroleum. Bioremediation was conducted in a closed bipolar system, by bioaugmentation with consortia of hydrocarbon degrading microorganisms (HD) and biostimulation with nutrients. Comprehensive twodimensional gas chromatography-mass spectrometry (GC X GC-MS) was used to visualize all fractions simultaneously. During the study, the content of total petroleum hydrocarbon (TPH) in groundwater decreased by 92.7% of the initial level, and the average rate of biodegradation was 0.1 mg/L per day. Increased numbers of HD were observed and the dominant genera were Pseudomonas, Rhodococcus, Achromobacter, Bacillus, and Micromonospora. In the first 30 days of bioremediation, there was no significant biodegradation of n-alkanes and petroleum biomarkers - isoprenoids such as pristane and phytane, and polycyclic-saturated hydrocarbons such as terpanes and steranes. However, after 60 days of bioremediation, more than 95% of n-alkanes, terpanes and steranes were biodegraded. Phenanthrene and its methyl-,dimethyl-, and trimethyl-isomers were biodegraded and reduced by more than 99% of their initial levels. However, their decomposition had clearly commenced after just 30 days. This is a somewhat surprising result since it follows that the phenanthrenes were more susceptible to biodegradation than the n-alkanes and isoprenoids. Depending on the microbial community used for bioaugmentation, biodegradation of phenanthrene can precede biodegradation of saturated hydrocarbons.", publisher = "Wiley, Hoboken", journal = "Clean - Soil, Air, Water", title = "Biodegradation of Isoprenoids, Steranes, Terpanes, and Phenanthrenes During In Situ Bioremediation of Petroleum-Contaminated Groundwater", volume = "45", number = "2", doi = "10.1002/clen.201600023" }
Beškoski, V., Miletić, S., Ilić, M., Gojgić-Cvijović, G., Papić, P., Marić, N., Šolević Knudsen, T., Jovančićević, B., Nakano, T.,& Vrvić, M.. (2017). Biodegradation of Isoprenoids, Steranes, Terpanes, and Phenanthrenes During In Situ Bioremediation of Petroleum-Contaminated Groundwater. in Clean - Soil, Air, Water Wiley, Hoboken., 45(2). https://doi.org/10.1002/clen.201600023
Beškoski V, Miletić S, Ilić M, Gojgić-Cvijović G, Papić P, Marić N, Šolević Knudsen T, Jovančićević B, Nakano T, Vrvić M. Biodegradation of Isoprenoids, Steranes, Terpanes, and Phenanthrenes During In Situ Bioremediation of Petroleum-Contaminated Groundwater. in Clean - Soil, Air, Water. 2017;45(2). doi:10.1002/clen.201600023 .
Beškoski, Vladimir, Miletić, Srđan, Ilić, Mila, Gojgić-Cvijović, Gordana, Papić, Petar, Marić, Nenad, Šolević Knudsen, Tatjana, Jovančićević, Branimir, Nakano, Takeshi, Vrvić, Miroslav, "Biodegradation of Isoprenoids, Steranes, Terpanes, and Phenanthrenes During In Situ Bioremediation of Petroleum-Contaminated Groundwater" in Clean - Soil, Air, Water, 45, no. 2 (2017), https://doi.org/10.1002/clen.201600023 . .