Identification of cytotoxic metabolites from Mahonia aquifolium using H-1 NMR-based metabolomics approach
Само за регистроване кориснике
2018
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Herein, we propose a H-1 NMR-based metabolomics method to reveal cytotoxic metabolites from Mahonia aquifolium stem-bark. Primary and secondary metabolites in the Mahonia aquifolium extracts were identified by thorough analysis of H-1 and 2D NMR spectra, without prior isolation. An OPLS multivariate analysis method was used to correlate the chemical composition of the plant extracts with the results of cytotoxic activity against Human cervical adenocarcinoma cell line. Protoberberine alkaloids berberine and palmatine, along with bisbenzylisoquinoline alkaloid berbamine were identified as the most influential in the OPLS model, with the highest cytotoxic activity.
Кључне речи:
Cytotoxic activity / NMR / Metabolomics / Mahonia aquifoliumИзвор:
Journal of Pharmaceutical and Biomedical Analysis, 2018, 150, 9-14Издавач:
- Elsevier
Финансирање / пројекти:
- Биоактивни природни производи самониклих, гајених и јестивих биљака: одређивање структура и активности (RS-MESTD-Basic Research (BR or ON)-172053)
- Модификатори биолошког одговора у физиолошким и патолошким стањима (RS-MESTD-Basic Research (BR or ON)-175011)
DOI: 10.1016/j.jpba.2017.11.075
ISSN: 0731-7085
PubMed: 29202306
WoS: 000423497000002
Scopus: 2-s2.0-85036522547
Институција/група
IHTMTY - JOUR AU - Gođevac, Dejan AU - Damjanovic, Ana AU - Stanojković, Tatjana AU - Anđelković, Boban D. AU - Zdunić, Gordana PY - 2018 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/2464 AB - Herein, we propose a H-1 NMR-based metabolomics method to reveal cytotoxic metabolites from Mahonia aquifolium stem-bark. Primary and secondary metabolites in the Mahonia aquifolium extracts were identified by thorough analysis of H-1 and 2D NMR spectra, without prior isolation. An OPLS multivariate analysis method was used to correlate the chemical composition of the plant extracts with the results of cytotoxic activity against Human cervical adenocarcinoma cell line. Protoberberine alkaloids berberine and palmatine, along with bisbenzylisoquinoline alkaloid berbamine were identified as the most influential in the OPLS model, with the highest cytotoxic activity. PB - Elsevier T2 - Journal of Pharmaceutical and Biomedical Analysis T1 - Identification of cytotoxic metabolites from Mahonia aquifolium using H-1 NMR-based metabolomics approach VL - 150 SP - 9 EP - 14 DO - 10.1016/j.jpba.2017.11.075 ER -
@article{ author = "Gođevac, Dejan and Damjanovic, Ana and Stanojković, Tatjana and Anđelković, Boban D. and Zdunić, Gordana", year = "2018", abstract = "Herein, we propose a H-1 NMR-based metabolomics method to reveal cytotoxic metabolites from Mahonia aquifolium stem-bark. Primary and secondary metabolites in the Mahonia aquifolium extracts were identified by thorough analysis of H-1 and 2D NMR spectra, without prior isolation. An OPLS multivariate analysis method was used to correlate the chemical composition of the plant extracts with the results of cytotoxic activity against Human cervical adenocarcinoma cell line. Protoberberine alkaloids berberine and palmatine, along with bisbenzylisoquinoline alkaloid berbamine were identified as the most influential in the OPLS model, with the highest cytotoxic activity.", publisher = "Elsevier", journal = "Journal of Pharmaceutical and Biomedical Analysis", title = "Identification of cytotoxic metabolites from Mahonia aquifolium using H-1 NMR-based metabolomics approach", volume = "150", pages = "9-14", doi = "10.1016/j.jpba.2017.11.075" }
Gođevac, D., Damjanovic, A., Stanojković, T., Anđelković, B. D.,& Zdunić, G.. (2018). Identification of cytotoxic metabolites from Mahonia aquifolium using H-1 NMR-based metabolomics approach. in Journal of Pharmaceutical and Biomedical Analysis Elsevier., 150, 9-14. https://doi.org/10.1016/j.jpba.2017.11.075
Gođevac D, Damjanovic A, Stanojković T, Anđelković BD, Zdunić G. Identification of cytotoxic metabolites from Mahonia aquifolium using H-1 NMR-based metabolomics approach. in Journal of Pharmaceutical and Biomedical Analysis. 2018;150:9-14. doi:10.1016/j.jpba.2017.11.075 .
Gođevac, Dejan, Damjanovic, Ana, Stanojković, Tatjana, Anđelković, Boban D., Zdunić, Gordana, "Identification of cytotoxic metabolites from Mahonia aquifolium using H-1 NMR-based metabolomics approach" in Journal of Pharmaceutical and Biomedical Analysis, 150 (2018):9-14, https://doi.org/10.1016/j.jpba.2017.11.075 . .