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(1->3)- β-D-glukan iz saccharomyces cerevisiae - dobijanje, karakterizacija i hemijska modifikacija uvođenjem karbonilnih grupa u polisaharidni niz

dc.creatorZeković, Đorđe
dc.creatorRadulović, Milanka
dc.creatorNastasović, Aleksandra
dc.creatorVrvić, Miroslav
dc.creatorJakovljević, Dragica
dc.date.accessioned2019-01-30T17:13:46Z
dc.date.available2019-01-30T17:13:46Z
dc.date.issued2006
dc.identifier.issn0581-1538
dc.identifier.urihttp://cer.ihtm.bg.ac.rs/handle/123456789/227
dc.description.abstractA number of polysaccharides with [3-glycosidic linkage are widespread in a variety of sources. All have a common structure, the (1->3)- β -D-glucan backbone is essential. They have attracted attention because of bioactive and medicinal properties which have been described for more than 50 years. Glucan from technologically important species, baker's yeast Saccharomyces cerevisiae. was isolated in a purified form and characterized. Data from common analytical methods supported by GLC-MS and NMR spectra indicated that isolated glucan from Saccharomyces cerevisiae has a backbone chain up of (l->3)- β -D-linked glucopyranoses with single β -D-glucopyranosul units at the branch points on every eight main chain unit. The oxidative transformation of β -D-glucan was investigated with dimethyl sulfoxide-acetic-anhydride reagent. Keto-aldehydo polymers were generated by treatment of β -D-glucan with this reagent. Accompanyng'the oxidation was the formation of (methylthio)methyl groups along the polysaccharide chain at a degree of substitution smaller than the degree of substitution for the carbonyl group. As these groups groups are acid-labile, it has been found that can be removed by acid hydrolysis under conditions that not affect on the carbonyl content.en
dc.description.abstractBrojni polisaharidi sa p-glikozidnom vezom rasprostranjeni su u različitim izvorima. Svi imaju opštu strukturu u kojoj je (l->3)- β -D-glukan suštinska gradivna jedinica. Oni privlače pažnju zato što su bioaktivni i imaju medicinske osobine, koje su bile opisane pre više od 50 godina. Cinkanje izolovan u čistom obliku i okarakterisan iz pekarskog kvasca Sacclwromyces cerevisiae kao tehnološki važnog proizvoda. Podaci dobijeni pomoću opštih analitičkih metoda i potvrdjeni GLC-MS i NMR spektrima, ukazuju da izolovani glukan iz Saccharomyces cerevisiae ima kao osnovni gradivni niz (l->3)- β -D-povezane glukopiranoze sa pojedinačnim β -D-glukopiranoznim jedinicama u tačkama račvanja na svakih osam jedinica glavnog lanca. Oksidativna transformacija β -D-glukana ispitivana je sa reagensom dimetil sulfoksid-anhidrid sirćetne kiseline, pri čemu su dobijeni keto-aldehido polimeri. Oksidaciju prati nastajanje (metiltio)metil grupa duž polisaharidnog niza, a stepen supstitucije je manji nego za karbonilnu grupu. Pošto su ove grupe acido-labilne, utvrdjeno je da mogu da se eliminišu kiselom hidrolizom, bez uticaja na sadržaj karbonila.sr
dc.rightsopenAccess
dc.sourceActa biologica iugoslavica - serija B: Mikrobiologija
dc.subjectglucanen
dc.subjectSaccharomyces cerevisiaeen
dc.subjectcharacterizationen
dc.subjectoxidationen
dc.subjectdimethyl sultbxide-acetic anhydride reagenten
dc.title(1->3)-β-D-glucan from saccharomyces cerevisiae, preparation, characterization and chemical modification: Introducing carbonyl groups into the polysaccharide chainen
dc.title(1->3)- β-D-glukan iz saccharomyces cerevisiae - dobijanje, karakterizacija i hemijska modifikacija uvođenjem karbonilnih grupa u polisaharidni nizsr
dc.typearticle
dc.rights.licenseARR
dcterms.abstractВрвић, Мирослав М.; Радуловић, Миланка; Јаковљевић, Драгица; Зековић, Ђорђе; Настасовић, Aлександра;
dc.citation.volume43
dc.citation.issue1
dc.citation.spage15
dc.citation.epage29
dc.citation.other43(1): 15-29
dc.identifier.rcubConv_481
dc.identifier.fulltexthttp://cer.ihtm.bg.ac.rs//bitstream/id/8655/225.pdf
dc.type.versionpublishedVersion


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