Determination of ionization constants (PKA) of β-hydroxy-β-arylalkanoic acids using high-pressure liquid chromatography
Abstract
pKa values of five β-hydroxy-β-arylalkanoic acids and ibuprofen were determined using the RP-HPLC method. Stationary phase was octadecyl modified (C-18) silica gel, and mobile phase was a mixture of methanol and one of nine different buffers (60:40, v/v). wspH values were measured after mixing methanol with an appropriate buffer. The mean retention time of each compound was plotted against wspH of each mobile phase. The inflection point of each sigmoidal curve represented wspK a of the compound. Using wspK a in already known equations for the specific methanol/buffer mixture, wwpK a values were calculated. Obtained pKa values for synthesized compounds were in a narrow range from 3.34-3.81 and pKa for ibuprofen was 4.45. Predicted pKa values for these compounds in SPARC software showed good correlation with experimental pKa values (R2=0.8048).
Keywords:
dissociation constant / anti-inflammatory compounds / ibuprofen / liquid chromatographySource:
Kragujevac Journal of Science, 2018, 40, 103-111Publisher:
- University of Kragujevac, Faculty of Science
Funding / projects:
Collections
Institution/Community
IHTMTY - JOUR AU - Savić, Jelena S. AU - Marković, Bojan AU - Vitnik, Vesna AU - Dilber, Sanda P. PY - 2018 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/2271 AB - pKa values of five β-hydroxy-β-arylalkanoic acids and ibuprofen were determined using the RP-HPLC method. Stationary phase was octadecyl modified (C-18) silica gel, and mobile phase was a mixture of methanol and one of nine different buffers (60:40, v/v). wspH values were measured after mixing methanol with an appropriate buffer. The mean retention time of each compound was plotted against wspH of each mobile phase. The inflection point of each sigmoidal curve represented wspK a of the compound. Using wspK a in already known equations for the specific methanol/buffer mixture, wwpK a values were calculated. Obtained pKa values for synthesized compounds were in a narrow range from 3.34-3.81 and pKa for ibuprofen was 4.45. Predicted pKa values for these compounds in SPARC software showed good correlation with experimental pKa values (R2=0.8048). PB - University of Kragujevac, Faculty of Science T2 - Kragujevac Journal of Science T1 - Determination of ionization constants (PKA) of β-hydroxy-β-arylalkanoic acids using high-pressure liquid chromatography IS - 40 SP - 103 EP - 111 DO - 10.5937/KgJSci1840103S ER -
@article{ author = "Savić, Jelena S. and Marković, Bojan and Vitnik, Vesna and Dilber, Sanda P.", year = "2018", abstract = "pKa values of five β-hydroxy-β-arylalkanoic acids and ibuprofen were determined using the RP-HPLC method. Stationary phase was octadecyl modified (C-18) silica gel, and mobile phase was a mixture of methanol and one of nine different buffers (60:40, v/v). wspH values were measured after mixing methanol with an appropriate buffer. The mean retention time of each compound was plotted against wspH of each mobile phase. The inflection point of each sigmoidal curve represented wspK a of the compound. Using wspK a in already known equations for the specific methanol/buffer mixture, wwpK a values were calculated. Obtained pKa values for synthesized compounds were in a narrow range from 3.34-3.81 and pKa for ibuprofen was 4.45. Predicted pKa values for these compounds in SPARC software showed good correlation with experimental pKa values (R2=0.8048).", publisher = "University of Kragujevac, Faculty of Science", journal = "Kragujevac Journal of Science", title = "Determination of ionization constants (PKA) of β-hydroxy-β-arylalkanoic acids using high-pressure liquid chromatography", number = "40", pages = "103-111", doi = "10.5937/KgJSci1840103S" }
Savić, J. S., Marković, B., Vitnik, V.,& Dilber, S. P.. (2018). Determination of ionization constants (PKA) of β-hydroxy-β-arylalkanoic acids using high-pressure liquid chromatography. in Kragujevac Journal of Science University of Kragujevac, Faculty of Science.(40), 103-111. https://doi.org/10.5937/KgJSci1840103S
Savić JS, Marković B, Vitnik V, Dilber SP. Determination of ionization constants (PKA) of β-hydroxy-β-arylalkanoic acids using high-pressure liquid chromatography. in Kragujevac Journal of Science. 2018;(40):103-111. doi:10.5937/KgJSci1840103S .
Savić, Jelena S., Marković, Bojan, Vitnik, Vesna, Dilber, Sanda P., "Determination of ionization constants (PKA) of β-hydroxy-β-arylalkanoic acids using high-pressure liquid chromatography" in Kragujevac Journal of Science, no. 40 (2018):103-111, https://doi.org/10.5937/KgJSci1840103S . .