Приказ основних података о документу
Selective anticancer activity of hydroxyapatite/chitosan-poly(D,L)-lactide-co-glycolide particles loaded with an androstane-based cancer inhibitor
dc.creator | Ignjatović, Nenad | |
dc.creator | Penov-Gasi, Katarina M. | |
dc.creator | Wu, Victoria M. | |
dc.creator | Ajduković, Jovana J. | |
dc.creator | Kojić, Vesna V. | |
dc.creator | Vasiljević-Radović, Dana | |
dc.creator | Kuzmanovic, Maja | |
dc.creator | Uskokovicć, Vuk | |
dc.creator | Uskoković, Dragan P. | |
dc.date.accessioned | 2019-01-30T17:51:17Z | |
dc.date.available | 2019-01-30T17:51:17Z | |
dc.date.issued | 2016 | |
dc.identifier.issn | 0927-7765 | |
dc.identifier.uri | https://cer.ihtm.bg.ac.rs/handle/123456789/1996 | |
dc.description.abstract | In an earlier study we demonstrated that hydroxyapatite nanoparticles coated with chitosan-poly(D,L)-lactide-co-glycolide (HAp/Ch-PLGA) target lungs following their intravenous injection into mice. In this study we utilize an emulsification process and freeze drying to load the composite HAp/Ch-PLGA particles with 17 beta-hydroxy-17 alpha-picolyl-androst-5-en-3 beta-yl-acetate (A), a chemotherapeutic derivative of androstane and a novel compound with a selective anticancer activity against lung cancer cells. H-1 NMR and C-13 NMR techniques confirmed the intact structure of the derivative A following its entrapment within HAp/Ch-PLGA particles. The thermogravimetric and differential thermal analyses coupled with mass spectrometry were used to assess the thermal degradation products and properties of A-loaded HAp/Ch-PLGA. The loading efficiency, as indicated by the comparison of enthalpies of phase transitions in pure A and A-loaded HAp/Ch-PLGA, equaled 7.47 wt.%. The release of A from HAp/Ch-PLGA was sustained, neither exhibiting a burst release nor plateauing after three weeks. Atomic force microscopy and particle size distribution analyses were used to confirm that the particles were spherical with a uniform size distribution of d(50) = 168 nm. In vitro cytotoxicity testing of A-loaded HAp/Ch-PLGA using MTT and trypan blue dye exclusion assays demonstrated that the particles were cytotoxic to the A549 human lung carcinoma cell line (46 +/- 2%), while simultaneously preserving high viability (83 +/- 3%) of regular MRC5 human lung fibroblasts and causing no harm to primary mouse lung fibroblasts. In conclusion, composite A-loaded HAp/Ch-PLGA particles could be seen as promising drug delivery platforms for selective cancer therapies, targeting malignant cells for destruction, while having a significantly lesser cytotoxic effect on the healthy cells. | en |
dc.publisher | Elsevier | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/45004/RS// | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172021/RS// | |
dc.relation | United States National Institutes of Health - R00-DE021416 | |
dc.rights | restrictedAccess | |
dc.source | Colloids and Surfaces B-Biointerfaces | |
dc.subject | Androstane | en |
dc.subject | Chitosan | en |
dc.subject | Hydroxyapatite | en |
dc.subject | Lung cancer | en |
dc.subject | Nanoparticle | en |
dc.subject | PLGA | en |
dc.title | Selective anticancer activity of hydroxyapatite/chitosan-poly(D,L)-lactide-co-glycolide particles loaded with an androstane-based cancer inhibitor | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Ускоковицћ, Вук; Ускоковић, Драган П.; Игњатовић, Ненад Л.; Пенов-Гаси, Катарина М.; Wу, Вицториа М.; Aјдуковић, Јована Ј.; Којиц, Весна В.; Васиљевић-Радовић, Дана; Кузмановиц, Маја; | |
dc.citation.volume | 148 | |
dc.citation.spage | 629 | |
dc.citation.epage | 639 | |
dc.citation.other | 148: 629-639 | |
dc.citation.rank | M21 | |
dc.identifier.pmid | 27694053 | |
dc.identifier.doi | 10.1016/j.colsurfb.2016.09.041 | |
dc.identifier.scopus | 2-s2.0-84989184184 | |
dc.identifier.wos | 000388248500073 | |
dc.type.version | publishedVersion |