Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose
Аутори
Jugović, BranimirGrgur, Branimir N.
Antov, Mirjana
Knežević-Jugović, Zorica
Stevanović, Jasmina
Gvozdenović, Milica M.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Polypyrrole enzyme electrode was formed by immobilization of glucose oxidase via glutaraldehyde into electrochemically synthesized polypyrrole on glassy carbon electrode. Electrochemical synthesis was performed in 0.5 mol dm(-3) HCl and 0.2 mol dm(-3) pyrrole at constant current density of 2 mA cm(-2). Chronopotentiometric curves of polypyrrole enzyme electrode were recorded at current density of 42 nA cm(-2) for different glucose concentrations. The determined value of the apparent Michaelis-Menten constant was 0.045 mmol dm(-3) which is significantly lower than that of free enzyme indicating enhanced enzyme efficiency when it is immobilized into electroconducting polymer matrix.
Кључне речи:
conducting polymers / electrochemistry / glucose oxidase / material testing / sensorsИзвор:
International Journal of Electrochemical Science, 2016, 11, 2, 1152-1161Издавач:
- Esg, Belgrade
Финансирање / пројекти:
- Електрохемијска синтеза и карактеризација наноструктурираних функционалних материјала за примену у новим технологијама (RS-MESTD-Basic Research (BR or ON)-172046)
Институција/група
IHTMTY - JOUR AU - Jugović, Branimir AU - Grgur, Branimir N. AU - Antov, Mirjana AU - Knežević-Jugović, Zorica AU - Stevanović, Jasmina AU - Gvozdenović, Milica M. PY - 2016 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/1962 AB - Polypyrrole enzyme electrode was formed by immobilization of glucose oxidase via glutaraldehyde into electrochemically synthesized polypyrrole on glassy carbon electrode. Electrochemical synthesis was performed in 0.5 mol dm(-3) HCl and 0.2 mol dm(-3) pyrrole at constant current density of 2 mA cm(-2). Chronopotentiometric curves of polypyrrole enzyme electrode were recorded at current density of 42 nA cm(-2) for different glucose concentrations. The determined value of the apparent Michaelis-Menten constant was 0.045 mmol dm(-3) which is significantly lower than that of free enzyme indicating enhanced enzyme efficiency when it is immobilized into electroconducting polymer matrix. PB - Esg, Belgrade T2 - International Journal of Electrochemical Science T1 - Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose VL - 11 IS - 2 SP - 1152 EP - 1161 UR - https://hdl.handle.net/21.15107/rcub_dais_15979 ER -
@article{ author = "Jugović, Branimir and Grgur, Branimir N. and Antov, Mirjana and Knežević-Jugović, Zorica and Stevanović, Jasmina and Gvozdenović, Milica M.", year = "2016", abstract = "Polypyrrole enzyme electrode was formed by immobilization of glucose oxidase via glutaraldehyde into electrochemically synthesized polypyrrole on glassy carbon electrode. Electrochemical synthesis was performed in 0.5 mol dm(-3) HCl and 0.2 mol dm(-3) pyrrole at constant current density of 2 mA cm(-2). Chronopotentiometric curves of polypyrrole enzyme electrode were recorded at current density of 42 nA cm(-2) for different glucose concentrations. The determined value of the apparent Michaelis-Menten constant was 0.045 mmol dm(-3) which is significantly lower than that of free enzyme indicating enhanced enzyme efficiency when it is immobilized into electroconducting polymer matrix.", publisher = "Esg, Belgrade", journal = "International Journal of Electrochemical Science", title = "Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose", volume = "11", number = "2", pages = "1152-1161", url = "https://hdl.handle.net/21.15107/rcub_dais_15979" }
Jugović, B., Grgur, B. N., Antov, M., Knežević-Jugović, Z., Stevanović, J.,& Gvozdenović, M. M.. (2016). Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose. in International Journal of Electrochemical Science Esg, Belgrade., 11(2), 1152-1161. https://hdl.handle.net/21.15107/rcub_dais_15979
Jugović B, Grgur BN, Antov M, Knežević-Jugović Z, Stevanović J, Gvozdenović MM. Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose. in International Journal of Electrochemical Science. 2016;11(2):1152-1161. https://hdl.handle.net/21.15107/rcub_dais_15979 .
Jugović, Branimir, Grgur, Branimir N., Antov, Mirjana, Knežević-Jugović, Zorica, Stevanović, Jasmina, Gvozdenović, Milica M., "Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose" in International Journal of Electrochemical Science, 11, no. 2 (2016):1152-1161, https://hdl.handle.net/21.15107/rcub_dais_15979 .