Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose
Authors
Jugović, BranimirGrgur, Branimir N.
Antov, Mirjana
Knežević-Jugović, Zorica
Stevanović, Jasmina
Gvozdenović, Milica M.
Article (Published version)
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Show full item recordAbstract
Polypyrrole enzyme electrode was formed by immobilization of glucose oxidase via glutaraldehyde into electrochemically synthesized polypyrrole on glassy carbon electrode. Electrochemical synthesis was performed in 0.5 mol dm(-3) HCl and 0.2 mol dm(-3) pyrrole at constant current density of 2 mA cm(-2). Chronopotentiometric curves of polypyrrole enzyme electrode were recorded at current density of 42 nA cm(-2) for different glucose concentrations. The determined value of the apparent Michaelis-Menten constant was 0.045 mmol dm(-3) which is significantly lower than that of free enzyme indicating enhanced enzyme efficiency when it is immobilized into electroconducting polymer matrix.
Keywords:
conducting polymers / electrochemistry / glucose oxidase / material testing / sensorsSource:
International Journal of Electrochemical Science, 2016, 11, 2, 1152-1161Publisher:
- Esg, Belgrade
Funding / projects:
- Electrochemical synthesis and characterization of nanostructured functional materials for application in new technologies (RS-MESTD-Basic Research (BR or ON)-172046)
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IHTMTY - JOUR AU - Jugović, Branimir AU - Grgur, Branimir N. AU - Antov, Mirjana AU - Knežević-Jugović, Zorica AU - Stevanović, Jasmina AU - Gvozdenović, Milica M. PY - 2016 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/1962 AB - Polypyrrole enzyme electrode was formed by immobilization of glucose oxidase via glutaraldehyde into electrochemically synthesized polypyrrole on glassy carbon electrode. Electrochemical synthesis was performed in 0.5 mol dm(-3) HCl and 0.2 mol dm(-3) pyrrole at constant current density of 2 mA cm(-2). Chronopotentiometric curves of polypyrrole enzyme electrode were recorded at current density of 42 nA cm(-2) for different glucose concentrations. The determined value of the apparent Michaelis-Menten constant was 0.045 mmol dm(-3) which is significantly lower than that of free enzyme indicating enhanced enzyme efficiency when it is immobilized into electroconducting polymer matrix. PB - Esg, Belgrade T2 - International Journal of Electrochemical Science T1 - Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose VL - 11 IS - 2 SP - 1152 EP - 1161 UR - https://hdl.handle.net/21.15107/rcub_dais_15979 ER -
@article{ author = "Jugović, Branimir and Grgur, Branimir N. and Antov, Mirjana and Knežević-Jugović, Zorica and Stevanović, Jasmina and Gvozdenović, Milica M.", year = "2016", abstract = "Polypyrrole enzyme electrode was formed by immobilization of glucose oxidase via glutaraldehyde into electrochemically synthesized polypyrrole on glassy carbon electrode. Electrochemical synthesis was performed in 0.5 mol dm(-3) HCl and 0.2 mol dm(-3) pyrrole at constant current density of 2 mA cm(-2). Chronopotentiometric curves of polypyrrole enzyme electrode were recorded at current density of 42 nA cm(-2) for different glucose concentrations. The determined value of the apparent Michaelis-Menten constant was 0.045 mmol dm(-3) which is significantly lower than that of free enzyme indicating enhanced enzyme efficiency when it is immobilized into electroconducting polymer matrix.", publisher = "Esg, Belgrade", journal = "International Journal of Electrochemical Science", title = "Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose", volume = "11", number = "2", pages = "1152-1161", url = "https://hdl.handle.net/21.15107/rcub_dais_15979" }
Jugović, B., Grgur, B. N., Antov, M., Knežević-Jugović, Z., Stevanović, J.,& Gvozdenović, M. M.. (2016). Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose. in International Journal of Electrochemical Science Esg, Belgrade., 11(2), 1152-1161. https://hdl.handle.net/21.15107/rcub_dais_15979
Jugović B, Grgur BN, Antov M, Knežević-Jugović Z, Stevanović J, Gvozdenović MM. Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose. in International Journal of Electrochemical Science. 2016;11(2):1152-1161. https://hdl.handle.net/21.15107/rcub_dais_15979 .
Jugović, Branimir, Grgur, Branimir N., Antov, Mirjana, Knežević-Jugović, Zorica, Stevanović, Jasmina, Gvozdenović, Milica M., "Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose" in International Journal of Electrochemical Science, 11, no. 2 (2016):1152-1161, https://hdl.handle.net/21.15107/rcub_dais_15979 .