Efficient stabilization of Saccharomyces cerevisiae external invertase by immobilisation on modified beidellite nanoclays
Samo za registrovane korisnike
2015
Autori
Anđelković, UrošMilutinović Nikolić, Aleksandra
Jović-Jovičić, Nataša
Banković, Predrag
Bajt, Teja
Mojović, Zorica
Vujčić, Zoran
Jovanović, Dušan M.
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The external invertase isoform 1 (EINV1) was immobilised on eight differently modified beidellite nanoclays, Modifications were composed of organo-modification with different amounts of surfactant - hexadecyl trimethylammonium cation (HDTMA), pillaring with Al/Fe containing polyhydroxy cations and acid modification of Na-enriched and pillared clays. The modified nanoclays were characterised by XRD, N-2-physisorption, SEM and FT-IR spectroscopy. The amount of bound enzyme activity was significantly influenced by the modification of beidellite ranging from 50 to remarkable 2200 U/g. Biochemical characterization was performed for five modified nanoclays showing the highest enzyme activity after invertase immobilisation. The investigation demonstrated that after immobilisation the structure and the catalytic properties of invertase were preserved, while Km values were slightly increased from 26 to 37 mM. immobilisation significantly improved thermal and storage stability of EINV1. Results ...indicate that beidellite nanoclays obtained by low cost modifications can be applied as a suitable support for the immobilisation of invertase. The immobilizate can be efficiently engaged in sucrose hydrolysis in batch reactor.
Ključne reči:
Invertase / Immobilisation / Beidellite / Sucrose hydrolysis / NanoclayIzvor:
Food Chemistry, 2015, 168, 262-269Izdavač:
- Elsevier Sci Ltd, Oxford
Finansiranje / projekti:
- Proizvodnja, izolovanje i karakterizacija enzima i malih molekula i njihova primena u rastvornom i imobilizovanom obliku u biotehnologiji hrane, biogorivima i zaštititi životne sredine (RS-172048)
- Nanostrukturni funkcionalni i kompozitni materijali u katalitičkim i sorpcionim procesima (RS-45001)
DOI: 10.1016/j.foodchem.2014.07.055
ISSN: 0308-8146
PubMed: 25172709
WoS: 000343612800035
Scopus: 2-s2.0-84904962782
Institucija/grupa
IHTMTY - JOUR AU - Anđelković, Uroš AU - Milutinović Nikolić, Aleksandra AU - Jović-Jovičić, Nataša AU - Banković, Predrag AU - Bajt, Teja AU - Mojović, Zorica AU - Vujčić, Zoran AU - Jovanović, Dušan M. PY - 2015 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/1728 AB - The external invertase isoform 1 (EINV1) was immobilised on eight differently modified beidellite nanoclays, Modifications were composed of organo-modification with different amounts of surfactant - hexadecyl trimethylammonium cation (HDTMA), pillaring with Al/Fe containing polyhydroxy cations and acid modification of Na-enriched and pillared clays. The modified nanoclays were characterised by XRD, N-2-physisorption, SEM and FT-IR spectroscopy. The amount of bound enzyme activity was significantly influenced by the modification of beidellite ranging from 50 to remarkable 2200 U/g. Biochemical characterization was performed for five modified nanoclays showing the highest enzyme activity after invertase immobilisation. The investigation demonstrated that after immobilisation the structure and the catalytic properties of invertase were preserved, while Km values were slightly increased from 26 to 37 mM. immobilisation significantly improved thermal and storage stability of EINV1. Results indicate that beidellite nanoclays obtained by low cost modifications can be applied as a suitable support for the immobilisation of invertase. The immobilizate can be efficiently engaged in sucrose hydrolysis in batch reactor. PB - Elsevier Sci Ltd, Oxford T2 - Food Chemistry T1 - Efficient stabilization of Saccharomyces cerevisiae external invertase by immobilisation on modified beidellite nanoclays VL - 168 SP - 262 EP - 269 DO - 10.1016/j.foodchem.2014.07.055 ER -
@article{ author = "Anđelković, Uroš and Milutinović Nikolić, Aleksandra and Jović-Jovičić, Nataša and Banković, Predrag and Bajt, Teja and Mojović, Zorica and Vujčić, Zoran and Jovanović, Dušan M.", year = "2015", abstract = "The external invertase isoform 1 (EINV1) was immobilised on eight differently modified beidellite nanoclays, Modifications were composed of organo-modification with different amounts of surfactant - hexadecyl trimethylammonium cation (HDTMA), pillaring with Al/Fe containing polyhydroxy cations and acid modification of Na-enriched and pillared clays. The modified nanoclays were characterised by XRD, N-2-physisorption, SEM and FT-IR spectroscopy. The amount of bound enzyme activity was significantly influenced by the modification of beidellite ranging from 50 to remarkable 2200 U/g. Biochemical characterization was performed for five modified nanoclays showing the highest enzyme activity after invertase immobilisation. The investigation demonstrated that after immobilisation the structure and the catalytic properties of invertase were preserved, while Km values were slightly increased from 26 to 37 mM. immobilisation significantly improved thermal and storage stability of EINV1. Results indicate that beidellite nanoclays obtained by low cost modifications can be applied as a suitable support for the immobilisation of invertase. The immobilizate can be efficiently engaged in sucrose hydrolysis in batch reactor.", publisher = "Elsevier Sci Ltd, Oxford", journal = "Food Chemistry", title = "Efficient stabilization of Saccharomyces cerevisiae external invertase by immobilisation on modified beidellite nanoclays", volume = "168", pages = "262-269", doi = "10.1016/j.foodchem.2014.07.055" }
Anđelković, U., Milutinović Nikolić, A., Jović-Jovičić, N., Banković, P., Bajt, T., Mojović, Z., Vujčić, Z.,& Jovanović, D. M.. (2015). Efficient stabilization of Saccharomyces cerevisiae external invertase by immobilisation on modified beidellite nanoclays. in Food Chemistry Elsevier Sci Ltd, Oxford., 168, 262-269. https://doi.org/10.1016/j.foodchem.2014.07.055
Anđelković U, Milutinović Nikolić A, Jović-Jovičić N, Banković P, Bajt T, Mojović Z, Vujčić Z, Jovanović DM. Efficient stabilization of Saccharomyces cerevisiae external invertase by immobilisation on modified beidellite nanoclays. in Food Chemistry. 2015;168:262-269. doi:10.1016/j.foodchem.2014.07.055 .
Anđelković, Uroš, Milutinović Nikolić, Aleksandra, Jović-Jovičić, Nataša, Banković, Predrag, Bajt, Teja, Mojović, Zorica, Vujčić, Zoran, Jovanović, Dušan M., "Efficient stabilization of Saccharomyces cerevisiae external invertase by immobilisation on modified beidellite nanoclays" in Food Chemistry, 168 (2015):262-269, https://doi.org/10.1016/j.foodchem.2014.07.055 . .