Efficient stabilization of Saccharomyces cerevisiae external invertase by immobilisation on modified beidellite nanoclays
Само за регистроване кориснике
2015
Аутори
Anđelković, UrošMilutinović Nikolić, Aleksandra
Jović-Jovičić, Nataša
Banković, Predrag
Bajt, Teja
Mojović, Zorica
Vujčić, Zoran
Jovanović, Dušan M.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The external invertase isoform 1 (EINV1) was immobilised on eight differently modified beidellite nanoclays, Modifications were composed of organo-modification with different amounts of surfactant - hexadecyl trimethylammonium cation (HDTMA), pillaring with Al/Fe containing polyhydroxy cations and acid modification of Na-enriched and pillared clays. The modified nanoclays were characterised by XRD, N-2-physisorption, SEM and FT-IR spectroscopy. The amount of bound enzyme activity was significantly influenced by the modification of beidellite ranging from 50 to remarkable 2200 U/g. Biochemical characterization was performed for five modified nanoclays showing the highest enzyme activity after invertase immobilisation. The investigation demonstrated that after immobilisation the structure and the catalytic properties of invertase were preserved, while Km values were slightly increased from 26 to 37 mM. immobilisation significantly improved thermal and storage stability of EINV1. Results ...indicate that beidellite nanoclays obtained by low cost modifications can be applied as a suitable support for the immobilisation of invertase. The immobilizate can be efficiently engaged in sucrose hydrolysis in batch reactor.
Кључне речи:
Invertase / Immobilisation / Beidellite / Sucrose hydrolysis / NanoclayИзвор:
Food Chemistry, 2015, 168, 262-269Издавач:
- Elsevier Sci Ltd, Oxford
Финансирање / пројекти:
- Производња, изоловање и карактеризација ензима и малих молекула и њихова примена у растворном и имобилизованом облику у биотехнологији хране, биогоривима и заштитити животне средине (RS-172048)
- Наноструктурни функционални и композитни материјали у каталитичким и сорпционим процесима (RS-45001)
DOI: 10.1016/j.foodchem.2014.07.055
ISSN: 0308-8146
PubMed: 25172709
WoS: 000343612800035
Scopus: 2-s2.0-84904962782
Институција/група
IHTMTY - JOUR AU - Anđelković, Uroš AU - Milutinović Nikolić, Aleksandra AU - Jović-Jovičić, Nataša AU - Banković, Predrag AU - Bajt, Teja AU - Mojović, Zorica AU - Vujčić, Zoran AU - Jovanović, Dušan M. PY - 2015 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/1728 AB - The external invertase isoform 1 (EINV1) was immobilised on eight differently modified beidellite nanoclays, Modifications were composed of organo-modification with different amounts of surfactant - hexadecyl trimethylammonium cation (HDTMA), pillaring with Al/Fe containing polyhydroxy cations and acid modification of Na-enriched and pillared clays. The modified nanoclays were characterised by XRD, N-2-physisorption, SEM and FT-IR spectroscopy. The amount of bound enzyme activity was significantly influenced by the modification of beidellite ranging from 50 to remarkable 2200 U/g. Biochemical characterization was performed for five modified nanoclays showing the highest enzyme activity after invertase immobilisation. The investigation demonstrated that after immobilisation the structure and the catalytic properties of invertase were preserved, while Km values were slightly increased from 26 to 37 mM. immobilisation significantly improved thermal and storage stability of EINV1. Results indicate that beidellite nanoclays obtained by low cost modifications can be applied as a suitable support for the immobilisation of invertase. The immobilizate can be efficiently engaged in sucrose hydrolysis in batch reactor. PB - Elsevier Sci Ltd, Oxford T2 - Food Chemistry T1 - Efficient stabilization of Saccharomyces cerevisiae external invertase by immobilisation on modified beidellite nanoclays VL - 168 SP - 262 EP - 269 DO - 10.1016/j.foodchem.2014.07.055 ER -
@article{ author = "Anđelković, Uroš and Milutinović Nikolić, Aleksandra and Jović-Jovičić, Nataša and Banković, Predrag and Bajt, Teja and Mojović, Zorica and Vujčić, Zoran and Jovanović, Dušan M.", year = "2015", abstract = "The external invertase isoform 1 (EINV1) was immobilised on eight differently modified beidellite nanoclays, Modifications were composed of organo-modification with different amounts of surfactant - hexadecyl trimethylammonium cation (HDTMA), pillaring with Al/Fe containing polyhydroxy cations and acid modification of Na-enriched and pillared clays. The modified nanoclays were characterised by XRD, N-2-physisorption, SEM and FT-IR spectroscopy. The amount of bound enzyme activity was significantly influenced by the modification of beidellite ranging from 50 to remarkable 2200 U/g. Biochemical characterization was performed for five modified nanoclays showing the highest enzyme activity after invertase immobilisation. The investigation demonstrated that after immobilisation the structure and the catalytic properties of invertase were preserved, while Km values were slightly increased from 26 to 37 mM. immobilisation significantly improved thermal and storage stability of EINV1. Results indicate that beidellite nanoclays obtained by low cost modifications can be applied as a suitable support for the immobilisation of invertase. The immobilizate can be efficiently engaged in sucrose hydrolysis in batch reactor.", publisher = "Elsevier Sci Ltd, Oxford", journal = "Food Chemistry", title = "Efficient stabilization of Saccharomyces cerevisiae external invertase by immobilisation on modified beidellite nanoclays", volume = "168", pages = "262-269", doi = "10.1016/j.foodchem.2014.07.055" }
Anđelković, U., Milutinović Nikolić, A., Jović-Jovičić, N., Banković, P., Bajt, T., Mojović, Z., Vujčić, Z.,& Jovanović, D. M.. (2015). Efficient stabilization of Saccharomyces cerevisiae external invertase by immobilisation on modified beidellite nanoclays. in Food Chemistry Elsevier Sci Ltd, Oxford., 168, 262-269. https://doi.org/10.1016/j.foodchem.2014.07.055
Anđelković U, Milutinović Nikolić A, Jović-Jovičić N, Banković P, Bajt T, Mojović Z, Vujčić Z, Jovanović DM. Efficient stabilization of Saccharomyces cerevisiae external invertase by immobilisation on modified beidellite nanoclays. in Food Chemistry. 2015;168:262-269. doi:10.1016/j.foodchem.2014.07.055 .
Anđelković, Uroš, Milutinović Nikolić, Aleksandra, Jović-Jovičić, Nataša, Banković, Predrag, Bajt, Teja, Mojović, Zorica, Vujčić, Zoran, Jovanović, Dušan M., "Efficient stabilization of Saccharomyces cerevisiae external invertase by immobilisation on modified beidellite nanoclays" in Food Chemistry, 168 (2015):262-269, https://doi.org/10.1016/j.foodchem.2014.07.055 . .