Spent mushroom compost as substrate for the production of industrially important hydrolytic enzymes by fungi Trichoderma spp. and Aspergillus niger in solid state fermentation
Само за регистроване кориснике
2015
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Mushroom production is the biggest solid state fermentation industry in the world. Disposal and storage of spent mushroom compost (SMC) that remains after mushroom harvest poses a big economic and environmental problem. Production of industrially important hydrolytic enzymes by fungi on various agro-industrial wastes is a significant, open chapter in biotechnology. This paper proposes a novel application of SMC as substrate for cultivation of fungi in solid state fermentation (SSF) in order to obtain the enzymes cellulase, xylanase, amylase and beta-glucosidase. SMC can be used as a good substrate for cultivation of Trichoderma and Aspergillus without the addition of supplementary (nutritive) elements. Starting amount of SMC was reduced by 30% due to hydrolysis by a complex of cellulolytic enzymes. Material that is left behind is a more suitable fertilizer for horticulture. One fungal isolate was pointed out as a promising producer (Trichoderma atroviride isolate T42). It produced the ...greatest amount of total protein (0.204 mg mL(-1)), five isoforms of beta-glucosidase and the highest level (12 isoforms) of both endocellulase (0.76 U mL(-1)) and xylanase (2.31 U mL(-1)). The capacity of T42 to produce all examined enzymes in such a high number of isoforms demonstrates successful adaptation to new substrates.
Кључне речи:
Spent mushroom compost / Trichoderma / Amylase / Cellulase / XylanaseИзвор:
International Biodeterioration & Biodegradation, 2015, 104, 290-298Издавач:
- Elsevier Sci Ltd, Oxford
Финансирање / пројекти:
- Производња, изоловање и карактеризација ензима и малих молекула и њихова примена у растворном и имобилизованом облику у биотехнологији хране, биогоривима и заштитити животне средине (RS-172048)
- Проучавање биљних патогена, артропода, корова и пестицида у циљу развоја метода биорационалне заштите биља и производње безбедне хране (RS-31043)
DOI: 10.1016/j.ibiod.2015.04.029
ISSN: 0964-8305
WoS: 000362308400041
Scopus: 2-s2.0-84934766303
Институција/група
IHTMTY - JOUR AU - Grujic, Marica AU - Dojnov, Biljana AU - Potocnik, Ivana AU - Duduk, Bojan AU - Vujčić, Zoran PY - 2015 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/1673 AB - Mushroom production is the biggest solid state fermentation industry in the world. Disposal and storage of spent mushroom compost (SMC) that remains after mushroom harvest poses a big economic and environmental problem. Production of industrially important hydrolytic enzymes by fungi on various agro-industrial wastes is a significant, open chapter in biotechnology. This paper proposes a novel application of SMC as substrate for cultivation of fungi in solid state fermentation (SSF) in order to obtain the enzymes cellulase, xylanase, amylase and beta-glucosidase. SMC can be used as a good substrate for cultivation of Trichoderma and Aspergillus without the addition of supplementary (nutritive) elements. Starting amount of SMC was reduced by 30% due to hydrolysis by a complex of cellulolytic enzymes. Material that is left behind is a more suitable fertilizer for horticulture. One fungal isolate was pointed out as a promising producer (Trichoderma atroviride isolate T42). It produced the greatest amount of total protein (0.204 mg mL(-1)), five isoforms of beta-glucosidase and the highest level (12 isoforms) of both endocellulase (0.76 U mL(-1)) and xylanase (2.31 U mL(-1)). The capacity of T42 to produce all examined enzymes in such a high number of isoforms demonstrates successful adaptation to new substrates. PB - Elsevier Sci Ltd, Oxford T2 - International Biodeterioration & Biodegradation T1 - Spent mushroom compost as substrate for the production of industrially important hydrolytic enzymes by fungi Trichoderma spp. and Aspergillus niger in solid state fermentation VL - 104 SP - 290 EP - 298 DO - 10.1016/j.ibiod.2015.04.029 ER -
@article{ author = "Grujic, Marica and Dojnov, Biljana and Potocnik, Ivana and Duduk, Bojan and Vujčić, Zoran", year = "2015", abstract = "Mushroom production is the biggest solid state fermentation industry in the world. Disposal and storage of spent mushroom compost (SMC) that remains after mushroom harvest poses a big economic and environmental problem. Production of industrially important hydrolytic enzymes by fungi on various agro-industrial wastes is a significant, open chapter in biotechnology. This paper proposes a novel application of SMC as substrate for cultivation of fungi in solid state fermentation (SSF) in order to obtain the enzymes cellulase, xylanase, amylase and beta-glucosidase. SMC can be used as a good substrate for cultivation of Trichoderma and Aspergillus without the addition of supplementary (nutritive) elements. Starting amount of SMC was reduced by 30% due to hydrolysis by a complex of cellulolytic enzymes. Material that is left behind is a more suitable fertilizer for horticulture. One fungal isolate was pointed out as a promising producer (Trichoderma atroviride isolate T42). It produced the greatest amount of total protein (0.204 mg mL(-1)), five isoforms of beta-glucosidase and the highest level (12 isoforms) of both endocellulase (0.76 U mL(-1)) and xylanase (2.31 U mL(-1)). The capacity of T42 to produce all examined enzymes in such a high number of isoforms demonstrates successful adaptation to new substrates.", publisher = "Elsevier Sci Ltd, Oxford", journal = "International Biodeterioration & Biodegradation", title = "Spent mushroom compost as substrate for the production of industrially important hydrolytic enzymes by fungi Trichoderma spp. and Aspergillus niger in solid state fermentation", volume = "104", pages = "290-298", doi = "10.1016/j.ibiod.2015.04.029" }
Grujic, M., Dojnov, B., Potocnik, I., Duduk, B.,& Vujčić, Z.. (2015). Spent mushroom compost as substrate for the production of industrially important hydrolytic enzymes by fungi Trichoderma spp. and Aspergillus niger in solid state fermentation. in International Biodeterioration & Biodegradation Elsevier Sci Ltd, Oxford., 104, 290-298. https://doi.org/10.1016/j.ibiod.2015.04.029
Grujic M, Dojnov B, Potocnik I, Duduk B, Vujčić Z. Spent mushroom compost as substrate for the production of industrially important hydrolytic enzymes by fungi Trichoderma spp. and Aspergillus niger in solid state fermentation. in International Biodeterioration & Biodegradation. 2015;104:290-298. doi:10.1016/j.ibiod.2015.04.029 .
Grujic, Marica, Dojnov, Biljana, Potocnik, Ivana, Duduk, Bojan, Vujčić, Zoran, "Spent mushroom compost as substrate for the production of industrially important hydrolytic enzymes by fungi Trichoderma spp. and Aspergillus niger in solid state fermentation" in International Biodeterioration & Biodegradation, 104 (2015):290-298, https://doi.org/10.1016/j.ibiod.2015.04.029 . .