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Molecular Characterization of Recombinant Mus a 5 Allergen from Banana Fruit
dc.creator | Mrkić, Ivan | |
dc.creator | Abughren, Mohamed | |
dc.creator | Nikolić, Jasna | |
dc.creator | Anđelković, Uroš | |
dc.creator | Vassilopoulou, Emilia | |
dc.creator | Sinaniotis, Athanassios | |
dc.creator | Petersen, Arnd | |
dc.creator | Papadopoulos, Nikolaos G. | |
dc.creator | Gavrović-Jankulović, Marija | |
dc.date.accessioned | 2019-01-30T17:42:35Z | |
dc.date.available | 2019-01-30T17:42:35Z | |
dc.date.issued | 2014 | |
dc.identifier.issn | 1073-6085 | |
dc.identifier.uri | https://cer.ihtm.bg.ac.rs/handle/123456789/1579 | |
dc.description.abstract | Allergy to banana fruit appears to have become an important cause of fruit allergy in Europe. Among five allergens that have been found, beta-1,3-glucanase denoted as Mus a 5 was identified as a candidate allergen for the component-resolved allergy diagnosis of banana allergy. Because of the variations in protein levels in banana fruit, in this study Mus a 5 was produced as a fusion protein with glutathione-S-transferase in Escherichia coli. The recombinant Mus a 5 was purified under native conditions by a combination of affinity, ion-exchange, and reversed phase chromatography. N-terminal sequence was confirmed by Edman degradation and 55 % of the primary structure was identified by mass fingerprint, while the secondary structure was assessed by circular dichroism spectroscopy. IgG reactivity of recombinant protein was shown in 2-D immunoblot with anti-Mus a 5 antibodies, while IgG and IgE binding to natural Mus a 5 was inhibited with the recombinant Mus a 5 in immunoblot inhibition test. IgE reactivity of recombinant Mus a 5 was shown in ELISA within a group of ten persons sensitized to banana fruit. Recombinant Mus a 5 is a novel reagent suitable for the component-resolved allergy diagnosis of banana allergy. | en |
dc.publisher | Humana Press Inc, Totowa | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172049/RS// | |
dc.relation | info:eu-repo/grantAgreement/EC/FP7/256716/EU// | |
dc.rights | restrictedAccess | |
dc.source | Molecular Biotechnology | |
dc.subject | Allergen | en |
dc.subject | Banana glucanase | en |
dc.subject | Food allergy | en |
dc.subject | IgE reactivity | en |
dc.subject | Mus a 5 | en |
dc.title | Molecular Characterization of Recombinant Mus a 5 Allergen from Banana Fruit | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Aбугхрен, Мохамед; Синаниотис, Aтханассиос; Aнђелковић, Урош; Мркиц, Иван; Николиц, Јасна; Пападопоулос, Николаос Г.; Гавровиц-Јанкуловиц, Марија; Вассилопоулоу, Емилиа; Петерсен, Aрнд; | |
dc.citation.volume | 56 | |
dc.citation.issue | 6 | |
dc.citation.spage | 498 | |
dc.citation.epage | 506 | |
dc.citation.other | 56(6): 498-506 | |
dc.citation.rank | M22 | |
dc.identifier.pmid | 24198217 | |
dc.identifier.doi | 10.1007/s12033-013-9719-8 | |
dc.identifier.scopus | 2-s2.0-84900792894 | |
dc.identifier.wos | 000335504400002 | |
dc.type.version | publishedVersion |