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Površinska karakterizacija, hemo- i citokompatibilnost segmentiranih poliuretana na bazi poli(dimetilsiloksana)

dc.creatorPergal, Marija
dc.creatorNestorov, Jelena
dc.creatorTovilović-Kovačević, Gordana
dc.creatorJovančić, Petar
dc.creatorPezo, Lato
dc.creatorVasiljević-Radović, Dana
dc.creatorĐonlagić, Jasna
dc.date.accessioned2019-01-30T17:40:00Z
dc.date.available2019-01-30T17:40:00Z
dc.date.issued2014
dc.identifier.issn0367-598X
dc.identifier.urihttp://cer.ihtm.bg.ac.rs/handle/123456789/1456
dc.description.abstractSegmented polyurethanes based on poly(dimethylsiloxane), currently used for biomedical applications, have sub-optimal biocompatibility which reduces their efficacy. Improving the endothelial cell attachment and blood-contacting properties of PDMS-based copolymers would substantially improve their clinical applications. We have studied the surface properties and in vitro biocompatibility of two series of segmented poly(urethane-dimethylsiloxane)s (SPU-PDMS) based on hydroxypropyl- and hydroxyethoxypropyl-terminated PDMS with potential applications in blood-contacting medical devices. SPU-PDMS copolymers were characterized by contact angle measurements, surface free energy determination (calculated using the van Oss-Chaudhury-Good and Owens-Wendt methods), and atomic force microscopy. The biocompatibility of copolymers was evaluated using an endothelial EA.hy926 cell line by direct contact assay, before and after pre-treatment of copolymers with multicomponent protein mixture, as well as by a competitive blood-protein adsorption assay. The obtained results suggested good blood compatibility of synthesized copolymers. All copolymers exhibited good resistance to fibrinogen adsorption and all favored albumin adsorption. Copolymers based on hydroxyethoxypropyl-PDMS had lower hydrophobicity, higher surface free energy and better microphase separation in comparison with hydroxypropyl-PDMS-based copolymers, which promoted better endothelial cell attachment and growth on the surface of these polymers as compared to hydroxypropyl-PDMS-based copolymers. The results showed that SPU-PDMS copolymers display good surface properties, depending on the type of soft PDMS segments, which can be tailored for biomedical application requirements such as biomedical devices for short- and long-term uses.en
dc.description.abstractSegmentirani poliuretani na bazi poli(dimetilsiloksana), koji se trenutno koriste u biomedicini, imaju biokompatibilnost ispod optimalne što smanjuje njihovu efikasnost. Poboljšavajući vezivanje endotelnih ćelija i svojstva kopolimera na bazi PDMS u dodiru sa krvlju značajno bi se poboljšala i proširila njihova klinička primena. U ovom radu su proučavana površinska svojstva i in vitro biokompatibilnost dve serije segmentiranih poli(uretan-dimetilsiloksana) (SPU-PDMS) na bazi hidroksipropil- i hidroksietoksipropil- PDMS pretpolimera sa potencijalnim primenama u medicinskim uredjajima u kontaktu sa krvlju. SPU-PDMS kopolimeri su karakterisani merenjem kontaktnih uglova, određivanjem površinske energije (izračunate prema van Oss-Chaudhury-Good i Owens-Wendt metodama), i mikroskopijom atomskih sila. Biokompatibilnost kopolimera je ispitivana primenom endotelnih EA.hy926 ćelija u direktnom kontaktu, pre i nakon pretretiranja kopolimera sa višekomponentnom smešom proteina, kao i pomoću kompetitivne adsorpcije proteina. Dobijeni rezultati su potvrdili da sintetisani kopolimeri imaju dobru kompatibilnost prema krvi. Svi sintetisani kopolimeri pokazivali su dobru otpornost prema adsorpciji fibrinogena i svi kopolimeri su favorizovali adsorpciju albumina. Kopolimeri na bazi hidroksietoksipropil-PDMS imali su manju hidrofobnost, veću površinsku energiju, i bolju mikrofaznu separaciju u poređenju sa kopolimerima na bazi hidroksipropil-PDMS, što je dovelo do boljeg vezivanja i rasta endotelnih ćelija na površini ovih polimera u poređenju sa kopolimerima na bazi hidroksipropil-PDMS. Rezultati su pokazali da SPU-PDMS kopolimeri prikazuju dobra površinska svojstva, zavisno od vrste mekih PDMS segmenata, koja se mogu prilagođavati zahtevima u biomedicini, kao što su biomedicinski uređaji za kratkoročnu i dugoročnu upotrebu.sr
dc.publisherAssociation of Chemical Engineers of Serbia
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172062/RS//
dc.rightsopenAccess
dc.sourceHemijska industrija
dc.subjectpolyurethanesen
dc.subjectsiloxanesen
dc.subjectsurface free energyen
dc.subjectcell adhesionen
dc.subjectprotein adsorptionen
dc.subjectPoliuretanisr
dc.subjectSiloksanisr
dc.subjectPovršinska energijasr
dc.subjectVezivanje ćelijasr
dc.subjectAdsorpcija proteinasr
dc.titleSurface characterization, hemo- and cytocompatibility of segmented poly(dimethylsiloxane)-based polyurethanesen
dc.titlePovršinska karakterizacija, hemo- i citokompatibilnost segmentiranih poliuretana na bazi poli(dimetilsiloksana)sr
dc.typearticle
dc.rights.licenseBY-NC-ND
dcterms.abstractПезо, Лато; Ђонлагић, Јасна; Јованчић, Петар; Несторов, Јелена; Пергал, Марија; Товиловић-Ковачевић, Гордана; Васиљевић-Радовић, Дана; Површинска карактеризација, хемо- и цитокомпатибилност сегментираних полиуретана на бази поли(диметилсилоксана); Површинска карактеризација, хемо- и цитокомпатибилност сегментираних полиуретана на бази поли(диметилсилоксана);
dc.citation.volume68
dc.citation.issue6
dc.citation.spage731
dc.citation.epage741
dc.citation.other68(6): 731-741
dc.citation.rankM23
dc.identifier.doi10.2298/HEMIND141103082P
dc.identifier.rcubConv_439
dc.identifier.fulltexthttp://cer.ihtm.bg.ac.rs//bitstream/id/7928/1454.pdf
dc.identifier.scopus2-s2.0-84920481537
dc.identifier.wos000348405900008
dc.type.versionpublishedVersion


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