Adsorption of lipase from Candida rugosa on multi walled carbon nanotubes
Samo za registrovane korisnike
2013
Autori
Prlainović, Nevena Ž.Bezbradica, Dejan I.
Knežević-Jugović, Zorica
Stevanović, Sanja
Avramov Ivić, Milka
Uskoković, Petar S.
Mijin, Dušan
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
In this work lipase from Candida rugosa was adsorbed on unmodified surface of multi walled carbon nanotubes (raw-MWCIsIT). The effects of immobilization time, initial enzyme concentration and buffer ionic strength on enzyme loading and activity of immobilized preparations were tested. High loadings are attained. The immobilized enzyme obtained at lowest initial enzyme concentration and high ionic strength retained 85% of initial enzyme activity. It is assumed that immobilization on hydrophobic surface led to conformational changes that resulted in the adsorption of lipase in active conformation. Immobilized preparations were characterized, with FT-IR spectroscopy, AFM, and cyclic voltammetry.
Ključne reči:
Lipase / Carbon nanotubes / Adsorption / FT-IR spectroscopy / AFM / Cyclic voltammetryIzvor:
Journal of Industrial and Engineering Chemistry, 2013, 19, 1, 279-285Izdavač:
- Korean Society of Industrial Engineering Chemistry
Finansiranje / projekti:
- Proučavanje sinteze, strukture i aktivnosti organskih jedinjenja prirodnog i sintetskog porekla (RS-172013)
- Razvoj novih inkapsulacionih i enzimskih tehnologija za proizvodnju biokatalizatora i biološki aktivnih komponenata hrane u cilju povećanja njene konkurentnosti, kvaliteta i bezbednosti (RS-46010)
- Sinteza, razvoj tehnologija dobijanja i primena nanostrukturnih multifunkcionalnih materijala definisanih svojstava (RS-45019)
DOI: 10.1016/j.jiec.2012.08.012
ISSN: 1226-086X
WoS: 000312764700039
Scopus: 2-s2.0-84870399209
Institucija/grupa
IHTMTY - JOUR AU - Prlainović, Nevena Ž. AU - Bezbradica, Dejan I. AU - Knežević-Jugović, Zorica AU - Stevanović, Sanja AU - Avramov Ivić, Milka AU - Uskoković, Petar S. AU - Mijin, Dušan PY - 2013 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/1297 AB - In this work lipase from Candida rugosa was adsorbed on unmodified surface of multi walled carbon nanotubes (raw-MWCIsIT). The effects of immobilization time, initial enzyme concentration and buffer ionic strength on enzyme loading and activity of immobilized preparations were tested. High loadings are attained. The immobilized enzyme obtained at lowest initial enzyme concentration and high ionic strength retained 85% of initial enzyme activity. It is assumed that immobilization on hydrophobic surface led to conformational changes that resulted in the adsorption of lipase in active conformation. Immobilized preparations were characterized, with FT-IR spectroscopy, AFM, and cyclic voltammetry. PB - Korean Society of Industrial Engineering Chemistry T2 - Journal of Industrial and Engineering Chemistry T1 - Adsorption of lipase from Candida rugosa on multi walled carbon nanotubes VL - 19 IS - 1 SP - 279 EP - 285 DO - 10.1016/j.jiec.2012.08.012 ER -
@article{ author = "Prlainović, Nevena Ž. and Bezbradica, Dejan I. and Knežević-Jugović, Zorica and Stevanović, Sanja and Avramov Ivić, Milka and Uskoković, Petar S. and Mijin, Dušan", year = "2013", abstract = "In this work lipase from Candida rugosa was adsorbed on unmodified surface of multi walled carbon nanotubes (raw-MWCIsIT). The effects of immobilization time, initial enzyme concentration and buffer ionic strength on enzyme loading and activity of immobilized preparations were tested. High loadings are attained. The immobilized enzyme obtained at lowest initial enzyme concentration and high ionic strength retained 85% of initial enzyme activity. It is assumed that immobilization on hydrophobic surface led to conformational changes that resulted in the adsorption of lipase in active conformation. Immobilized preparations were characterized, with FT-IR spectroscopy, AFM, and cyclic voltammetry.", publisher = "Korean Society of Industrial Engineering Chemistry", journal = "Journal of Industrial and Engineering Chemistry", title = "Adsorption of lipase from Candida rugosa on multi walled carbon nanotubes", volume = "19", number = "1", pages = "279-285", doi = "10.1016/j.jiec.2012.08.012" }
Prlainović, N. Ž., Bezbradica, D. I., Knežević-Jugović, Z., Stevanović, S., Avramov Ivić, M., Uskoković, P. S.,& Mijin, D.. (2013). Adsorption of lipase from Candida rugosa on multi walled carbon nanotubes. in Journal of Industrial and Engineering Chemistry Korean Society of Industrial Engineering Chemistry., 19(1), 279-285. https://doi.org/10.1016/j.jiec.2012.08.012
Prlainović NŽ, Bezbradica DI, Knežević-Jugović Z, Stevanović S, Avramov Ivić M, Uskoković PS, Mijin D. Adsorption of lipase from Candida rugosa on multi walled carbon nanotubes. in Journal of Industrial and Engineering Chemistry. 2013;19(1):279-285. doi:10.1016/j.jiec.2012.08.012 .
Prlainović, Nevena Ž., Bezbradica, Dejan I., Knežević-Jugović, Zorica, Stevanović, Sanja, Avramov Ivić, Milka, Uskoković, Petar S., Mijin, Dušan, "Adsorption of lipase from Candida rugosa on multi walled carbon nanotubes" in Journal of Industrial and Engineering Chemistry, 19, no. 1 (2013):279-285, https://doi.org/10.1016/j.jiec.2012.08.012 . .