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dc.creatorGorjanovic, Stanislava
dc.creatorPastor, Ferenc T.
dc.creatorVasic, Radica
dc.creatorNovaković, Miroslav
dc.creatorSimonović, Mladen
dc.creatorMilic, Sonja
dc.creatorSuznjevic, Desanka
dc.date.accessioned2019-01-30T17:35:26Z
dc.date.available2019-01-30T17:35:26Z
dc.date.issued2013
dc.identifier.issn0021-8561
dc.identifier.urihttp://cer.ihtm.bg.ac.rs/handle/123456789/1239
dc.description.abstractAntioxidant (AO) activity of extracts of hop cones (Serbian domestic varieties) and commercial hop products (Saaz, Spalter, Spalter select, and Magnum pellets) was determined by parallel application of recently developed direct current (DC) polarographic and widely used DPPH assay. Correlations between 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) scavenging and total phenolics (TPC) determined by the Folin-Ciocalteu assay (FC) (0.99), and between H2O2 scavenging,: (HPS) and humulone content (H) determined by conductometric method (0.94), total resins (TR) (0.85), and hop storage index (HIS) (-0.90), were found statistically significant at p LT 0.05 level while complete lack of HPS correlation with TPC and DPPH was observed. To obtain an insight into differences between results of AO assays applied, activity of individual compounds, prevalent hop phenolics, and bitter acids was determined. By far superior HPS activity of humulone was followed by catechin, quercetin, xanthohumol, lupulone, and rutin. In contrast, DPPH scavenging activity of phenolics (quercetin > catechin > rutin > xantohumol) was found substantially higher than activity of bitter acids. According to ferric reducing antioxidant power (FRAP) and scavenging of 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), higher AO activity was ascribed to phenolics, while almost neglecting humulone. Besides reliability, low cost, and an easy-to-handle procedure, an ability to recognize humulone as the major contributor of hop AO activity could allow DC polarographic assay to be applied in analysis of various hop-derived products.en
dc.publisherAmer Chemical Soc, Washington
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/43010/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31093/RS//
dc.rightsrestrictedAccess
dc.sourceJournal of Agricultural and Food Chemistry
dc.subjectantioxidant activityen
dc.subjecthopen
dc.subjecthydrogen peroxideen
dc.subjecthumuloneen
dc.subjectlupuloneen
dc.subjectphenolicsen
dc.subjectpolarographyen
dc.titleElectrochemical versus Spectrophotometric Assessment of Antioxidant Activity of Hop (Humulus lupulus L.) Products and Individual Compoundsen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractГорјановиц, Станислава; Милиц, Соња; Васиц, Радица; Симоновиц, Младен; Сузњевиц, Десанка; Новаковић, Мирослав; Пастор, Ференц Т.;
dc.citation.volume61
dc.citation.issue38
dc.citation.spage9089
dc.citation.epage9096
dc.citation.other61(38): 9089-9096
dc.citation.rankaM21
dc.identifier.pmid23971792
dc.identifier.doi10.1021/jf401718z
dc.identifier.rcubConv_3102
dc.identifier.scopus2-s2.0-84884852384
dc.identifier.wos000330096400011
dc.type.versionpublishedVersion


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