Antioxidant activity of propolis extracts from Serbia: A polarographic approach
Authorized Users Only
AuthorsPotkonjak, Nebojsa I.
Veselinovic, Dragan S.
Gorjanovic, Stanislava Z.
Suznjevic, Desanka Z.
Article (Published version)
MetadataShow full item record
Antioxidant activity (AO) of commercial propolis extracts (PEs), available on Serbian market, was determined by direct current (DC) polarography. Polarographic anodic current of 5.0 mmol L-1 alkaline solution of H2O2 was recorded at potentials of mercury dissolution. Decrease of the current was plotted against the volume of gradually added PEs. The volume of PE causing 20% current decrease was determined from the linear part of the plot. Antioxidant activity was expressed in H2O2 equivalent (HPEq), representing the volume of PE that corresponds to 1.0 mmol L-1 H2O2 decrease. Resulting HPEq ranged between 1.71 +/- 0.11 and 8.00 +/- 0.18 mu L. Range of 1,1-dipheny1-2-picryl hydrazyl (DPPH) radical scavenging activity was from 0.093 +/- 0.004% to 0.346 +/- 0.006%. Total phenolic content (TCP) of PE with superior AO activity was 5.31 +/- 0.05%g GAE, while the extract with the lowest activity contained 1.45 +/- 0.02%g GAE. Antioxidant activity, determined by polarographic method, was correl...ated with DPPH scavenging activity (R-2 = 0.991) and TCP (R-2 = 0.985). Validity of obtained results was further confirmed using ANOVA and post hoc Tukey HSD test.
Keywords:Propolis extract / Antioxidant activity / Hydrogen peroxide / Polarography
Source:Food and Chemical Toxicology, 2012, 50, 10, 3614-3618
- Pergamon-Elsevier Science Ltd, Oxford
- Dynamics of nonlinear physicochemical and biochemical systems with modeling and predicting of their behavior under nonequilibrium conditions (RS-172015)
- Modulation of antioxidative metabolism in plants for improvement of plant abiotic stress tolerance and identification of new biomarkers for application in remediation and monitoring of degraded biotopes (RS-43010)