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dc.creatorAleksić, Ivana
dc.creatorPopovic, Milica
dc.creatorDimitrijević, Rajna
dc.creatorAnđelković, Uroš
dc.creatorVassilopoulou, Emilia
dc.creatorSinaniotis, Athanassios
dc.creatorAtanasković-Marković, Marina
dc.creatorLindner, Buko
dc.creatorPetersen, Arnd
dc.creatorPapadopoulos, Nikolaos G.
dc.creatorGavrovic-Jankulovic, Marija
dc.date.accessioned2019-01-30T17:30:49Z
dc.date.available2019-01-30T17:30:49Z
dc.date.issued2012
dc.identifier.issn1613-4125
dc.identifier.urihttp://cer.ihtm.bg.ac.rs/handle/123456789/1024
dc.description.abstractScope Banana fruit has become an important cause of fruit allergy in the recent years. Among the five registered IUIS allergens, Mus a 1 and Mus a 2 have been characterized in detail. In this study, molecular characterization and evaluation of the allergenic properties of beta-1,3-glucanase from banana (Musa acuminata), denoted as Mus a 5, were performed Methods and results: The gene of Mus a 5 was cloned and sequenced. The obtained cDNA revealed a novel Mus a 5 isoform with an open reading frame encoding a protein of 340 amino acids comprising a putative signal peptide of 28 amino acid residues. By MALDI-TOF analysis Mus a 5 isolated from banana fruit revealed a molecular mass of 33 451 +/- 67 Da. Two Mus a 5 isoforms (pI 7.7 and 8.0) were detected by 2D immunoblot with an identical N-terminal sequence. By mass fingerprint, 76 and 83% of the primary structure was confirmed for the two mature Mus a 5 isoforms, respectively. IgE reactivity to Mus a 5 was found in 74% of patients sensitized to banana fruit. Upregulation of basophil activation markers CD63 and CD203c was achieved with Mus a 5 in a concentration-dependent manner. Conclusion: Mus a 5 is a functional allergen and a candidate for the component-resolved allergy diagnosis of banana allergy.en
dc.publisherWiley-Blackwell, Malden
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172049/RS//
dc.rightsrestrictedAccess
dc.sourceMolecular Nutrition & Food Research
dc.subjectAllergenen
dc.subjectBananaen
dc.subjectFood allergyen
dc.subjectGlucanaseen
dc.subjectMus a 5en
dc.titleMolecular and immunological characterization of Mus a 5 allergen from banana fruiten
dc.typearticle
dc.rights.licenseARR
dcterms.abstractAнђелковић, Урош; Димитријевић, Рајна; Вассилопоулоу, Емилиа; Aлексић, Ивана; Поповиц, Милица; Пападопоулос, Николаос Г.; Петерсен, Aрнд; Линднер, Буко; Aтанасковић-Марковић, Марина; Синаниотис, Aтханассиос; Гавровиц-Јанкуловиц, Марија;
dc.citation.volume56
dc.citation.issue3
dc.citation.spage446
dc.citation.epage453
dc.citation.other56(3): 446-453
dc.citation.rankaM21
dc.identifier.pmid22162266
dc.identifier.doi10.1002/mnfr.201100541
dc.identifier.rcubConv_2773
dc.identifier.scopus2-s2.0-84859181170
dc.identifier.wos000302169000008
dc.type.versionpublishedVersion


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