TY  - JOUR
AU  - Pergal, Marija
AU  - Nestorov, Jelena
AU  - Tovilović-Kovačević, Gordana
AU  - Jovančić, Petar
AU  - Pezo, Lato
AU  - Vasiljević-Radović, Dana
AU  - Đonlagić, Jasna
PY  - 2014
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1456
AB  - Segmented polyurethanes based on poly(dimethylsiloxane), currently used for biomedical applications, have sub-optimal biocompatibility which reduces their efficacy. Improving the endothelial cell attachment and blood-contacting properties of PDMS-based copolymers would substantially improve their clinical applications. We have studied the surface properties and in vitro biocompatibility of two series of segmented poly(urethane-dimethylsiloxane)s (SPU-PDMS) based on hydroxypropyl- and hydroxyethoxypropyl-terminated PDMS with potential applications in blood-contacting medical devices. SPU-PDMS copolymers were characterized by contact angle measurements, surface free energy determination (calculated using the van Oss-Chaudhury-Good and Owens-Wendt methods), and atomic force microscopy. The biocompatibility of copolymers was evaluated using an endothelial EA.hy926 cell line by direct contact assay, before and after pre-treatment of copolymers with multicomponent protein mixture, as well as by a competitive blood-protein adsorption assay. The obtained results suggested good blood compatibility of synthesized copolymers. All copolymers exhibited good resistance to fibrinogen adsorption and all favored albumin adsorption. Copolymers based on hydroxyethoxypropyl-PDMS had lower hydrophobicity, higher surface free energy and better microphase separation in comparison with hydroxypropyl-PDMS-based copolymers, which promoted better endothelial cell attachment and growth on the surface of these polymers as compared to hydroxypropyl-PDMS-based copolymers. The results showed that SPU-PDMS copolymers display good surface properties, depending on the type of soft PDMS segments, which can be tailored for biomedical application requirements such as biomedical devices for short- and long-term uses.
AB  - Segmentirani poliuretani na bazi poli(dimetilsiloksana), koji se trenutno koriste u biomedicini, imaju biokompatibilnost ispod optimalne što smanjuje njihovu efikasnost. Poboljšavajući vezivanje endotelnih ćelija i svojstva kopolimera na bazi PDMS u dodiru sa krvlju značajno bi se poboljšala i proširila njihova klinička primena. U ovom radu su proučavana površinska svojstva i in vitro biokompatibilnost dve serije segmentiranih poli(uretan-dimetilsiloksana) (SPU-PDMS) na bazi hidroksipropil- i hidroksietoksipropil- PDMS pretpolimera sa potencijalnim primenama u medicinskim uredjajima u kontaktu sa krvlju. SPU-PDMS kopolimeri su karakterisani merenjem kontaktnih uglova, određivanjem površinske energije (izračunate prema van Oss-Chaudhury-Good i Owens-Wendt metodama), i mikroskopijom atomskih sila. Biokompatibilnost kopolimera je ispitivana primenom endotelnih EA.hy926 ćelija u direktnom kontaktu, pre i nakon pretretiranja kopolimera sa višekomponentnom smešom proteina, kao i pomoću kompetitivne adsorpcije proteina. Dobijeni rezultati su potvrdili da sintetisani kopolimeri imaju dobru kompatibilnost prema krvi. Svi sintetisani kopolimeri pokazivali su dobru otpornost prema adsorpciji fibrinogena i svi kopolimeri su favorizovali adsorpciju albumina. Kopolimeri na bazi hidroksietoksipropil-PDMS imali su manju hidrofobnost, veću površinsku energiju, i bolju mikrofaznu separaciju u poređenju sa kopolimerima na bazi hidroksipropil-PDMS, što je dovelo do boljeg vezivanja i rasta endotelnih ćelija na površini ovih polimera u poređenju sa kopolimerima na bazi hidroksipropil-PDMS. Rezultati su pokazali da SPU-PDMS kopolimeri prikazuju dobra površinska svojstva, zavisno od vrste mekih PDMS segmenata, koja se mogu prilagođavati zahtevima u biomedicini, kao što su biomedicinski uređaji za kratkoročnu i dugoročnu upotrebu.
PB  - Association of Chemical Engineers of Serbia
T2  - Hemijska industrija
T1  - Surface characterization, hemo- and cytocompatibility of segmented poly(dimethylsiloxane)-based polyurethanes
T1  - Površinska karakterizacija, hemo- i citokompatibilnost segmentiranih poliuretana na bazi poli(dimetilsiloksana)
VL  - 68
IS  - 6
SP  - 731
EP  - 741
DO  - 10.2298/HEMIND141103082P
ER  - 

@article{
author = "Pergal, Marija and Nestorov, Jelena and Tovilović-Kovačević, Gordana and Jovančić, Petar and Pezo, Lato and Vasiljević-Radović, Dana and Đonlagić, Jasna",
year = "2014",
abstract = "Segmented polyurethanes based on poly(dimethylsiloxane), currently used for biomedical applications, have sub-optimal biocompatibility which reduces their efficacy. Improving the endothelial cell attachment and blood-contacting properties of PDMS-based copolymers would substantially improve their clinical applications. We have studied the surface properties and in vitro biocompatibility of two series of segmented poly(urethane-dimethylsiloxane)s (SPU-PDMS) based on hydroxypropyl- and hydroxyethoxypropyl-terminated PDMS with potential applications in blood-contacting medical devices. SPU-PDMS copolymers were characterized by contact angle measurements, surface free energy determination (calculated using the van Oss-Chaudhury-Good and Owens-Wendt methods), and atomic force microscopy. The biocompatibility of copolymers was evaluated using an endothelial EA.hy926 cell line by direct contact assay, before and after pre-treatment of copolymers with multicomponent protein mixture, as well as by a competitive blood-protein adsorption assay. The obtained results suggested good blood compatibility of synthesized copolymers. All copolymers exhibited good resistance to fibrinogen adsorption and all favored albumin adsorption. Copolymers based on hydroxyethoxypropyl-PDMS had lower hydrophobicity, higher surface free energy and better microphase separation in comparison with hydroxypropyl-PDMS-based copolymers, which promoted better endothelial cell attachment and growth on the surface of these polymers as compared to hydroxypropyl-PDMS-based copolymers. The results showed that SPU-PDMS copolymers display good surface properties, depending on the type of soft PDMS segments, which can be tailored for biomedical application requirements such as biomedical devices for short- and long-term uses., Segmentirani poliuretani na bazi poli(dimetilsiloksana), koji se trenutno koriste u biomedicini, imaju biokompatibilnost ispod optimalne što smanjuje njihovu efikasnost. Poboljšavajući vezivanje endotelnih ćelija i svojstva kopolimera na bazi PDMS u dodiru sa krvlju značajno bi se poboljšala i proširila njihova klinička primena. U ovom radu su proučavana površinska svojstva i in vitro biokompatibilnost dve serije segmentiranih poli(uretan-dimetilsiloksana) (SPU-PDMS) na bazi hidroksipropil- i hidroksietoksipropil- PDMS pretpolimera sa potencijalnim primenama u medicinskim uredjajima u kontaktu sa krvlju. SPU-PDMS kopolimeri su karakterisani merenjem kontaktnih uglova, određivanjem površinske energije (izračunate prema van Oss-Chaudhury-Good i Owens-Wendt metodama), i mikroskopijom atomskih sila. Biokompatibilnost kopolimera je ispitivana primenom endotelnih EA.hy926 ćelija u direktnom kontaktu, pre i nakon pretretiranja kopolimera sa višekomponentnom smešom proteina, kao i pomoću kompetitivne adsorpcije proteina. Dobijeni rezultati su potvrdili da sintetisani kopolimeri imaju dobru kompatibilnost prema krvi. Svi sintetisani kopolimeri pokazivali su dobru otpornost prema adsorpciji fibrinogena i svi kopolimeri su favorizovali adsorpciju albumina. Kopolimeri na bazi hidroksietoksipropil-PDMS imali su manju hidrofobnost, veću površinsku energiju, i bolju mikrofaznu separaciju u poređenju sa kopolimerima na bazi hidroksipropil-PDMS, što je dovelo do boljeg vezivanja i rasta endotelnih ćelija na površini ovih polimera u poređenju sa kopolimerima na bazi hidroksipropil-PDMS. Rezultati su pokazali da SPU-PDMS kopolimeri prikazuju dobra površinska svojstva, zavisno od vrste mekih PDMS segmenata, koja se mogu prilagođavati zahtevima u biomedicini, kao što su biomedicinski uređaji za kratkoročnu i dugoročnu upotrebu.",
publisher = "Association of Chemical Engineers of Serbia",
journal = "Hemijska industrija",
title = "Surface characterization, hemo- and cytocompatibility of segmented poly(dimethylsiloxane)-based polyurethanes, Površinska karakterizacija, hemo- i citokompatibilnost segmentiranih poliuretana na bazi poli(dimetilsiloksana)",
volume = "68",
number = "6",
pages = "731-741",
doi = "10.2298/HEMIND141103082P"
}

Pergal, M., Nestorov, J., Tovilović-Kovačević, G., Jovančić, P., Pezo, L., Vasiljević-Radović, D.,& Đonlagić, J.. (2014). Surface characterization, hemo- and cytocompatibility of segmented poly(dimethylsiloxane)-based polyurethanes. in Hemijska industrija
Association of Chemical Engineers of Serbia., 68(6), 731-741.
https://doi.org/10.2298/HEMIND141103082P

Pergal M, Nestorov J, Tovilović-Kovačević G, Jovančić P, Pezo L, Vasiljević-Radović D, Đonlagić J. Surface characterization, hemo- and cytocompatibility of segmented poly(dimethylsiloxane)-based polyurethanes. in Hemijska industrija. 2014;68(6):731-741.
doi:10.2298/HEMIND141103082P .

Pergal, Marija, Nestorov, Jelena, Tovilović-Kovačević, Gordana, Jovančić, Petar, Pezo, Lato, Vasiljević-Radović, Dana, Đonlagić, Jasna, "Surface characterization, hemo- and cytocompatibility of segmented poly(dimethylsiloxane)-based polyurethanes" in Hemijska industrija, 68, no. 6 (2014):731-741,
https://doi.org/10.2298/HEMIND141103082P . .

TY  - JOUR
AU  - Ilić, Vesna
AU  - Šaponjić, Zoran
AU  - Vodnik, Vesna
AU  - Potkonjak, Branislav
AU  - Jovančić, Petar
AU  - Nedeljković, Jovan
AU  - Radetić, Maja
PY  - 2009
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4080
AB  - The aim of this study was to examine the antimicrobial efficiency and color changes of cotton fabrics
loaded with colloidal silver nanoparticles which were synthesized without using any stabilizer. The influence
of colloidal concentration and consequently, the amount of silver deposited onto the fabric surface,
on antimicrobial activity against Gram-negative bacterium Escherichia coli, Gram-positive bacterium
Staphylococcus aureus and fungus Candida albicans as well as laundering durability of obtained effects
were studied. Although cotton fabrics loaded with silver nanoparticles from 10 ppm colloid exhibited
good antimicrobial efficiency, their poor laundering durability indicated that higher concentrated colloids
(50 ppm) must be applied for obtaining long-term durability. Additionally, the influence of dyeing with
C.I. Direct Red 81 on antimicrobial activity of cotton fabrics loaded with silver nanoparticles as well as the
influence of their presence on the color change of dyed fabrics were evaluated. Unlike color change, the
antimicrobial efficiency was not affected by the order of dyeing and loading of silver nanoparticles.
PB  - Elsevier
T2  - Carbohydrate Polymers
T1  - The influence of silver content on antimicrobial activity and color of cotton fabrics functionalized with Ag nanoparticles
VL  - 78
IS  - 3
SP  - 564
EP  - 569
DO  - 10.1016/j.carbpol.2009.05.015
ER  - 

@article{
author = "Ilić, Vesna and Šaponjić, Zoran and Vodnik, Vesna and Potkonjak, Branislav and Jovančić, Petar and Nedeljković, Jovan and Radetić, Maja",
year = "2009",
abstract = "The aim of this study was to examine the antimicrobial efficiency and color changes of cotton fabrics
loaded with colloidal silver nanoparticles which were synthesized without using any stabilizer. The influence
of colloidal concentration and consequently, the amount of silver deposited onto the fabric surface,
on antimicrobial activity against Gram-negative bacterium Escherichia coli, Gram-positive bacterium
Staphylococcus aureus and fungus Candida albicans as well as laundering durability of obtained effects
were studied. Although cotton fabrics loaded with silver nanoparticles from 10 ppm colloid exhibited
good antimicrobial efficiency, their poor laundering durability indicated that higher concentrated colloids
(50 ppm) must be applied for obtaining long-term durability. Additionally, the influence of dyeing with
C.I. Direct Red 81 on antimicrobial activity of cotton fabrics loaded with silver nanoparticles as well as the
influence of their presence on the color change of dyed fabrics were evaluated. Unlike color change, the
antimicrobial efficiency was not affected by the order of dyeing and loading of silver nanoparticles.",
publisher = "Elsevier",
journal = "Carbohydrate Polymers",
title = "The influence of silver content on antimicrobial activity and color of cotton fabrics functionalized with Ag nanoparticles",
volume = "78",
number = "3",
pages = "564-569",
doi = "10.1016/j.carbpol.2009.05.015"
}

Ilić, V., Šaponjić, Z., Vodnik, V., Potkonjak, B., Jovančić, P., Nedeljković, J.,& Radetić, M.. (2009). The influence of silver content on antimicrobial activity and color of cotton fabrics functionalized with Ag nanoparticles. in Carbohydrate Polymers
Elsevier., 78(3), 564-569.
https://doi.org/10.1016/j.carbpol.2009.05.015

Ilić V, Šaponjić Z, Vodnik V, Potkonjak B, Jovančić P, Nedeljković J, Radetić M. The influence of silver content on antimicrobial activity and color of cotton fabrics functionalized with Ag nanoparticles. in Carbohydrate Polymers. 2009;78(3):564-569.
doi:10.1016/j.carbpol.2009.05.015 .

Ilić, Vesna, Šaponjić, Zoran, Vodnik, Vesna, Potkonjak, Branislav, Jovančić, Petar, Nedeljković, Jovan, Radetić, Maja, "The influence of silver content on antimicrobial activity and color of cotton fabrics functionalized with Ag nanoparticles" in Carbohydrate Polymers, 78, no. 3 (2009):564-569,
https://doi.org/10.1016/j.carbpol.2009.05.015 . .

TY  - JOUR
AU  - Radetić, Maja
AU  - Radojević, Darinka
AU  - Ilić, Vesna
AU  - Povrenović, Dragan
AU  - Potkonjak, Branislav
AU  - Puač, Nevena
AU  - Jovančić, Petar M.
PY  - 2007
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4260
AB  - In this study, the effect of low-temperature air plasma, biopolymer chitosan
and hydrogen peroxide treatment of recycled wool-based non-woven material
on metal cation uptake was investigated. Recycled wool-based material either as an
untreated or modified material showed ability to bind all investigated metal cations
in the following order: Pb2+>Cu2+>Zn2+>Co2+. Material performed good selectivity
due to distinct sorption rates of studied metal cations.
AB  - U ovom radu je ispitan uticaj obrade netkanog materijala na bazi vune kao
sekundarne sirovine niskotemperaturnom plazmom, biopolimerom hitozanom i vodonik-
peroksidom na sorpciju katjona metala. Materijal na bazi vune kao sekundarne
sirovine bilo kao neobra|en ili modifikovan, poseduje sposobnost vezivawa katjona
metala prema slede}em redosledu: Pb2+>Cu2+>Zn2+>Co2+. Materijal pokazuje dobru
selektivnost usled razli~ite brzine sorpcije ispitivanih katjona metala.
PB  - Belgrade : Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - Removal of metal cations from wastewater using recycled wool-based non-woven material
T1  - Уклањање катјона метала из отпадне воде нетканим материјалом на бази вуне као секундарне сировине
VL  - 72
IS  - 6
SP  - 605
EP  - 614
DO  - 10.2298/JSC0706605R
ER  - 

@article{
author = "Radetić, Maja and Radojević, Darinka and Ilić, Vesna and Povrenović, Dragan and Potkonjak, Branislav and Puač, Nevena and Jovančić, Petar M.",
year = "2007",
abstract = "In this study, the effect of low-temperature air plasma, biopolymer chitosan
and hydrogen peroxide treatment of recycled wool-based non-woven material
on metal cation uptake was investigated. Recycled wool-based material either as an
untreated or modified material showed ability to bind all investigated metal cations
in the following order: Pb2+>Cu2+>Zn2+>Co2+. Material performed good selectivity
due to distinct sorption rates of studied metal cations., U ovom radu je ispitan uticaj obrade netkanog materijala na bazi vune kao
sekundarne sirovine niskotemperaturnom plazmom, biopolimerom hitozanom i vodonik-
peroksidom na sorpciju katjona metala. Materijal na bazi vune kao sekundarne
sirovine bilo kao neobra|en ili modifikovan, poseduje sposobnost vezivawa katjona
metala prema slede}em redosledu: Pb2+>Cu2+>Zn2+>Co2+. Materijal pokazuje dobru
selektivnost usled razli~ite brzine sorpcije ispitivanih katjona metala.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "Removal of metal cations from wastewater using recycled wool-based non-woven material, Уклањање катјона метала из отпадне воде нетканим материјалом на бази вуне као секундарне сировине",
volume = "72",
number = "6",
pages = "605-614",
doi = "10.2298/JSC0706605R"
}

Radetić, M., Radojević, D., Ilić, V., Povrenović, D., Potkonjak, B., Puač, N.,& Jovančić, P. M.. (2007). Removal of metal cations from wastewater using recycled wool-based non-woven material. in Journal of the Serbian Chemical Society
Belgrade : Serbian Chemical Society., 72(6), 605-614.
https://doi.org/10.2298/JSC0706605R

Radetić M, Radojević D, Ilić V, Povrenović D, Potkonjak B, Puač N, Jovančić PM. Removal of metal cations from wastewater using recycled wool-based non-woven material. in Journal of the Serbian Chemical Society. 2007;72(6):605-614.
doi:10.2298/JSC0706605R .

Radetić, Maja, Radojević, Darinka, Ilić, Vesna, Povrenović, Dragan, Potkonjak, Branislav, Puač, Nevena, Jovančić, Petar M., "Removal of metal cations from wastewater using recycled wool-based non-woven material" in Journal of the Serbian Chemical Society, 72, no. 6 (2007):605-614,
https://doi.org/10.2298/JSC0706605R . .